High mobility group box 1 and TLR4 signaling pathway in gnotobiotic piglets colonized/infected with L. amylovorus, L. mucosae, E. coli Nissle 1917 and S. typhimurium
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F19%3AN0000141" target="_blank" >RIV/60460709:41210/19:N0000141 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/19:00519835 RIV/68378050:_____/19:00519835 RIV/67985904:_____/19:00519835
Result on the web
<a href="https://www.mdpi.com/1422-0067/20/24/6294" target="_blank" >https://www.mdpi.com/1422-0067/20/24/6294</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms20246294" target="_blank" >10.3390/ijms20246294</a>
Alternative languages
Result language
angličtina
Original language name
High mobility group box 1 and TLR4 signaling pathway in gnotobiotic piglets colonized/infected with L. amylovorus, L. mucosae, E. coli Nissle 1917 and S. typhimurium
Original language description
High mobility group box 1 (HMGB1) is a DNA binding nuclear protein that can be actively secreted by immune cells after different immune stimuli or passively released from cells undergoing necrosis. HMGB1 amplifies inflammation, and its hypersecretion contributes to multiple organ dysfunction syndrome and death. We tested possible immunomodulatory effect of commensal Lactobacillus amylovorus (LA), Lactobacillus mucosae (LM) or probiotic Escherichia coli Nissle 1917 (EcN) in infection of gnotobiotic piglets with Salmonella Typhimurium (ST). Transcription of HMGB1 and Toll like receptors (TLR) 2, 4, and 9 and receptor for advanced glycation end products (RAGE), TLR4 related molecules (MD2, CD14, and LBP), and adaptor proteins (MyD88 and TRIF) in the ileum and colon were measured by RT qPCR. Expression of TLR4 and its related molecules were highly upregulated in the ST infected intestine, which was suppressed by EcN, but not LA nor LM. In contrast, HMGB1 expression was unaffected by ST infection or commensal probiotic administration. HMGB1 protein levels in the intestine measured by ELISA were increased in ST infected piglets, but they were decreased by previous colonization with E. coli Nissle 1917 only. We conclude that the stability of HMGB1 mRNA expression in all piglet groups could show its importance for DNA transcription and physiological cell functions. The presence of HMGB1 protein in the intestinal lumen probably indicates cellular damage.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/EF16_019%2F0000845" target="_blank" >EF16_019/0000845: Centre for investigation of synthesis and transformation of nutritional substances in the food chain in interaction with potentially harmful substances of athropogenic origin: assessment of contamination risks for the quality of production</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Molecular Sciences
ISSN
1422-0067
e-ISSN
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Volume of the periodical
20
Issue of the periodical within the volume
24
Country of publishing house
CH - SWITZERLAND
Number of pages
20
Pages from-to
1-20
UT code for WoS article
000506840100187
EID of the result in the Scopus database
2-s2.0-85076856092