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High mobility group box 1 and TLR4 signaling pathway in gnotobiotic piglets colonized/infected with L. amylovorus, L. mucosae, E. coli Nissle 1917 and S. typhimurium

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F19%3AN0000141" target="_blank" >RIV/60460709:41210/19:N0000141 - isvavai.cz</a>

  • Alternative codes found

    RIV/61388971:_____/19:00519835 RIV/68378050:_____/19:00519835 RIV/67985904:_____/19:00519835

  • Result on the web

    <a href="https://www.mdpi.com/1422-0067/20/24/6294" target="_blank" >https://www.mdpi.com/1422-0067/20/24/6294</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.3390/ijms20246294" target="_blank" >10.3390/ijms20246294</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    High mobility group box 1 and TLR4 signaling pathway in gnotobiotic piglets colonized/infected with L. amylovorus, L. mucosae, E. coli Nissle 1917 and S. typhimurium

  • Original language description

    High mobility group box 1 (HMGB1) is a DNA binding nuclear protein that can be actively secreted by immune cells after different immune stimuli or passively released from cells undergoing necrosis. HMGB1 amplifies inflammation, and its hypersecretion contributes to multiple organ dysfunction syndrome and death. We tested possible immunomodulatory effect of commensal Lactobacillus amylovorus (LA), Lactobacillus mucosae (LM) or probiotic Escherichia coli Nissle 1917 (EcN) in infection of gnotobiotic piglets with Salmonella Typhimurium (ST). Transcription of HMGB1 and Toll like receptors (TLR) 2, 4, and 9 and receptor for advanced glycation end products (RAGE), TLR4 related molecules (MD2, CD14, and LBP), and adaptor proteins (MyD88 and TRIF) in the ileum and colon were measured by RT qPCR. Expression of TLR4 and its related molecules were highly upregulated in the ST infected intestine, which was suppressed by EcN, but not LA nor LM. In contrast, HMGB1 expression was unaffected by ST infection or commensal probiotic administration. HMGB1 protein levels in the intestine measured by ELISA were increased in ST infected piglets, but they were decreased by previous colonization with E. coli Nissle 1917 only. We conclude that the stability of HMGB1 mRNA expression in all piglet groups could show its importance for DNA transcription and physiological cell functions. The presence of HMGB1 protein in the intestinal lumen probably indicates cellular damage.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10606 - Microbiology

Result continuities

  • Project

    <a href="/en/project/EF16_019%2F0000845" target="_blank" >EF16_019/0000845: Centre for investigation of synthesis and transformation of nutritional substances in the food chain in interaction with potentially harmful substances of athropogenic origin: assessment of contamination risks for the quality of production</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    International Journal of Molecular Sciences

  • ISSN

    1422-0067

  • e-ISSN

  • Volume of the periodical

    20

  • Issue of the periodical within the volume

    24

  • Country of publishing house

    CH - SWITZERLAND

  • Number of pages

    20

  • Pages from-to

    1-20

  • UT code for WoS article

    000506840100187

  • EID of the result in the Scopus database

    2-s2.0-85076856092