Pathogenic profile and cytotoxic activity of Aeromonas spp. isolated from Pectinatella magnifica and surrounding water in the South Bohemian aquaculture region
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F20%3AN0000109" target="_blank" >RIV/60460709:41210/20:N0000109 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/epdf/10.1111/jfd.13223" target="_blank" >https://onlinelibrary.wiley.com/doi/epdf/10.1111/jfd.13223</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/jfd.13223" target="_blank" >10.1111/jfd.13223</a>
Alternative languages
Result language
angličtina
Original language name
Pathogenic profile and cytotoxic activity of Aeromonas spp. isolated from Pectinatella magnifica and surrounding water in the South Bohemian aquaculture region
Original language description
Pectinatella magnifica is an invasive freshwater bryozoan that has expanded in many localities worldwide, including fishing areas. It contains microbial communities, pre- dominantly consisting of Aeromonas bacteria that are frequently associated with fish infections. The objective of this study was to investigate the potential pathogenicity of Aeromonas spp. associated with P. magnifica and evaluate the health risks for fish. Aeromonas strains were isolated from P. magnifica (101 strains) and from surrounding water (29 strains) in the South Bohemian region and investigated for the presence of 14 virulence-associated genes using PCR. We demonstrated high prevalence of phospholipase GCAT, polar flagellin, enolase, DNAse, aerolysin/cytotoxic entero- toxin, serine protease and heat-stable cytotonic enterotoxin-coding genes. Further, all twelve isolates that were analysed for cytotoxicity against intestinal epithelial cells were found to be cytotoxic. Six of the isolates were also tested as co-cultures com- posed of pairs. Enhanced cytotoxicity was observed when the pair was composed of strains from different species. In conclusion, P. magnifica is colonized by Aeromonas strains that have a relatively high prevalence of virulence-associated genes and the ability to provoke disease. Results also suggest a possibly increased risk arising from mixed infections.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/EF16_019%2F0000845" target="_blank" >EF16_019/0000845: Centre for investigation of synthesis and transformation of nutritional substances in the food chain in interaction with potentially harmful substances of athropogenic origin: assessment of contamination risks for the quality of production</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
JOURNAL OF FISH DISEASES
ISSN
1365-2761
e-ISSN
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Volume of the periodical
43
Issue of the periodical within the volume
10
Country of publishing house
GB - UNITED KINGDOM
Number of pages
15
Pages from-to
1213-1227
UT code for WoS article
000557396400001
EID of the result in the Scopus database
2-s2.0-85089136711