Molecular characterization of Aeromonas spp. isolates carrying plasmid-mediated quinolone resistance genes from carp and rainbow trout fish in Czech Republic
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F19%3A43877963" target="_blank" >RIV/62157124:16270/19:43877963 - isvavai.cz</a>
Result on the web
<a href="https://docplayer.cz/147890435-Xxi-konference-mladych-vedeckych-pracovniku-s-mezinarodni-ucasti-sbornik-prispevku.html" target="_blank" >https://docplayer.cz/147890435-Xxi-konference-mladych-vedeckych-pracovniku-s-mezinarodni-ucasti-sbornik-prispevku.html</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Molecular characterization of Aeromonas spp. isolates carrying plasmid-mediated quinolone resistance genes from carp and rainbow trout fish in Czech Republic
Original language description
Quinolone resistance in bacteria have been reported on a global scale and it is also associated with aquatic environments, prominently found in aeromonads. The aim of this study was to isolate and characterize Aeromonas spp. isolates with reduced susceptibility to ciprofloxacin in food fish (carp and rainbow trout fish) bred in the Czech Republic, and to assess their potential risks as sources of plasmid-mediated resistance to fluoroquinolones. Thirty six qnrS2-positive Aeromonas spp. isolates were speciated by sequencing of rpoB gene. Isolates originating from carps belonged to Aeromonas veronii while isolates from rainbow trout were identified as Aeromonas salmonicida. Susceptibility testing revealed that all selected isolates were resistant to oxolinic acid with a majority of isolates resistant to fluemequine and oxytetracycline. PCR-based detection showed that at least 2 virulence genes were associated with these isolates. Whole genome sequencing revealed that qnrS2 gene was located within a mobile insertion cassette and carried by a small mobilizable plasmid or larger IncU plasmids. A. veronii had low virulence with high diversity of genetic profile while A. salmonicida had at least 5 virulence genes and belonged to sequence type (ST)2. This study revealed the dissemination of transferrable quinolone resistance in fish pathogens in Czech fisheries.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
XXI. KONFERENCE MLADÝCH VĚDECKÝCH PRACOVNÍKŮ S MEZINÁRODNÍ ÚČASTÍ, Sborník příspěvků
ISBN
978-80-7305-820-3
ISSN
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e-ISSN
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Number of pages
3
Pages from-to
71-73
Publisher name
Veterinární a farmaceutická univerzita Brno
Place of publication
Brno
Event location
Brno
Event date
May 22, 2019
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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