Sensitive Fluorescence In Situ Hybridization on Semithin Sections of Adult Schistosoma mansoni Using DIG-Labeled RNA Probes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F20%3AN0000145" target="_blank" >RIV/60460709:41210/20:N0000145 - isvavai.cz</a>
Result on the web
<a href="https://experiments.springernature.com/articles/10.1007/978-1-0716-0635-3_4" target="_blank" >https://experiments.springernature.com/articles/10.1007/978-1-0716-0635-3_4</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Sensitive Fluorescence In Situ Hybridization on Semithin Sections of Adult Schistosoma mansoni Using DIG-Labeled RNA Probes
Original language description
In situ hybridization is a tool for evaluation of gene expression within tissues or single cells. This protocol describes optimized sensitive fluorescence detection of gene transcripts in semithin sections of Schistosoma mansoni adult worms using specifically designed and labeled RNA probes. Due to improved methodologies in tissue preservation, sectioning, amplification of fluorescent signal, and prehybridization tissue treatment, it is possible to detect transcripts in the fine structures of schistosomes. The protocol is sensitive enough to detect very low abundance targets. This procedure is optimized for tissues derived from S. mansoni adult worms, however, it can be successfully applied to other trematode species. Methods In Molecular Biology, Schistosoma mansoni Methods and Protocols. Humana Press, Springer Science. Totowa, New Jersey, United States. p265. ISBN 978-1-0716-0635-3. DOI 10.1007/978-1-0716-0635-3_4
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
30310 - Parasitology
Result continuities
Project
<a href="/en/project/GA18-14167S" target="_blank" >GA18-14167S: Orthologs of glutamate carboxypeptidase 2 in model organisms - search for physiological roles and therapeutic potential of the enigmatic enzyme</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů