Sensitive Fluorescence In Situ Hybridization on Semithin Sections of Adult Schistosoma mansoni Using DIG-Labeled RNA Probes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F20%3A00525099" target="_blank" >RIV/61388963:_____/20:00525099 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/20:10448302
Result on the web
<a href="http://dx.doi.org/10.1007/978-1-0716-0635-3_4" target="_blank" >http://dx.doi.org/10.1007/978-1-0716-0635-3_4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-0716-0635-3_4" target="_blank" >10.1007/978-1-0716-0635-3_4</a>
Alternative languages
Result language
angličtina
Original language name
Sensitive Fluorescence In Situ Hybridization on Semithin Sections of Adult Schistosoma mansoni Using DIG-Labeled RNA Probes
Original language description
In situ hybridization is a tool for evaluation of gene expression within tissues or single cells. This protocol describes optimized sensitive fluorescence detection of gene transcripts (mRNAs) in semithin sections of Schistosoma mansoni adult worms using specifically designed and labeled RNA probes. Due to improved methodologies in tissue preservation, sectioning, amplification of fluorescent signal, and prehybridization tissue treatment, it is possible to detect transcripts in the fine structures of schistosomes. The protocol is sensitive enough to detect very low abundance targets. This procedure is optimized for tissues derived from S. mansoni adult worms, however, it can be successfully applied to other trematode species.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/NV18-05-00345" target="_blank" >NV18-05-00345: Exopeptidase inhibitors as drugs against schistosomiasis: structure-based rational design, synthesis and functional characterization</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Schistosoma mansoni: Methods and Protocols
ISBN
978-1-0716-0634-6
Number of pages of the result
11
Pages from-to
43-53
Number of pages of the book
265
Publisher name
Humana Press
Place of publication
New York
UT code for WoS chapter
000680910400005