Reference Gene Selection for Normalizing Gene Expression in Ips Sexdentatus (Coleoptera: Curculionidae: Scolytinae) Under Different Experimental Conditions

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F21%3A88335" target="_blank" >RIV/60460709:41210/21:88335 - isvavai.cz</a>
Alternative codes found
RIV/60460709:41320/21:88335
Result on the web
<a href="https://www.frontiersin.org/articles/10.3389/fphys.2021.752768/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fphys.2021.752768/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fphys.2021.752768" target="_blank" >10.3389/fphys.2021.752768</a>

Result language
angličtina
Original language name
Reference Gene Selection for Normalizing Gene Expression in Ips Sexdentatus (Coleoptera: Curculionidae: Scolytinae) Under Different Experimental Conditions
Original language description
Ips sexdentatus is one of the most destructive and economically important forest pests. A better understanding of molecular mechanisms underlying its adaptation to toxic host compounds may unleash the potential for future management of this pest. A suitable reference gene selection is fundamental for quantitative gene expression analysis and functional genomics studies in I. sexdentatus. Twelve commonly used reference genes in Coleopterans were screened under different experimental conditions to obtain accurate and reliable normalization of gene expression data. The findings were validated further using real-time quantitative reverse transcription PCR (RT-qPCR)-based target gene expression analysis. Nevertheless, the present study delivers a catalog of reference genes under varied experimental conditions for the coleopteran forest pest I. sexdentatus and paves the way for future gene expression and functional genomic studies on this species.
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
40102 - Forestry

Project
<a href="/en/project/EF15_003%2F0000433" target="_blank" >EF15_003/0000433: Building up an excellent scientific team and its spatio-technical background focused on mitigation of the impact of climatic changes to forests from the level of a gene to the level of a landscape at the FFWS CULS Prague</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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