Detection of sex in adults and larvae of Leptinotarsa decemlineata on principle of copy number variation

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F22%3A91077" target="_blank" >RIV/60460709:41210/22:91077 - isvavai.cz</a>
Alternative codes found
RIV/60109807:_____/22:N0000003
Result on the web
<a href="https://www.nature.com/articles/s41598-022-08642-x" target="_blank" >https://www.nature.com/articles/s41598-022-08642-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-022-08642-x" target="_blank" >10.1038/s41598-022-08642-x</a>

Result language
angličtina
Original language name
Detection of sex in adults and larvae of Leptinotarsa decemlineata on principle of copy number variation
Original language description
The identification of sex in larvae of insects is usually challenging or even impossible, while in adults the sexual dimorphism is usually evident. Here, we used copy number analysis to develop a method of sex detection in Colorado potato beetle (Leptinotarsa decemlineata), which has an X0 sex determination system. The X linked gene LdVssc and autosomal gene LdUBE3B were identified as appropriate target and reference loci, respectively. The copy numbers (CNV) of LdVssc in males and females were estimated using standard droplet digital PCR (ddPCR) and realtime PCR (qPCR). With both methods, CNVs were bimodally distributed (BAddPCR = 0,709 and BAqPCR = 0,683) with 100% ability to distinguish females from males. The use of qPCR based sex detection in a broad collection of 448 random CPB adults showed a perfect association (Phi = 1,0, p less 0,05) with the true sexes of adults, with mean CNV in females of 2,032 (SD = 0,227) and 0,989 in males (SD = 0,147). In the collection of 50 random 4th instar larvae
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10620 - Other biological topics

Project
<a href="/en/project/QK1910270" target="_blank" >QK1910270: Innovation of integrated potato protection against the Colorado potato beetle based on new knowledge of genetic and biological characteristics</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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