Effect of Different Thawing Regimes on Cell Kinematics and Organelle Integrity of Nitrogen-Stored Wallachian Ram Spermatozoa

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F24%3A100953" target="_blank" >RIV/60460709:41210/24:100953 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/2306-7381/11/12/602" target="_blank" >https://www.mdpi.com/2306-7381/11/12/602</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/vetsci11120602" target="_blank" >10.3390/vetsci11120602</a>

Result language

angličtina

Original language name

Effect of Different Thawing Regimes on Cell Kinematics and Organelle Integrity of Nitrogen-Stored Wallachian Ram Spermatozoa

Original language description

It is well known that the suboptimal thawing of frozen insemination doses critically reduces the fertilizing ability of spermatozoa and, consequently, the effectiveness of artificial insemination. In this study, we aimed to determine the optimal thawing procedure for frozen Wallachian ram insemination doses. The study used a standard method of obtaining ejaculates from rams, diluting semen with a commercial extender, and a common methodology for freezing sperm in straws with minor modifications. After storage in liquid nitrogen, doses were thawed using several regimes: straws were thawed at 45, 50, 55, 60, and 65 degrees C for durations of 2, 5, 8, 11, and 14 s for each temperature. The thawing regime at 39 degrees for 30 s was chosen as a reference thawing method, to which all others were compared. Using a computer-assisted sperm analyser and flow cytometry, the motility, kinematic parameters, and spermatozoa cell organelle damage of the thawed samples were analysed and supplemented with an analysis of the internal temperature gradient and thermal energy absorption of each straw. Based on the obtained results, we concluded that the thawing regime at 39 degrees for 30 s is the preferred regime for ram sperm. Our study may be useful with regard to standardizing the procedure for the thawing of frozen ram sperm insemination doses and, therefore, might significantly improve the effectiveness of artificial insemination techniques in the sheep industry.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40200 - Animal and Dairy science

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Veterinary Sciences

ISSN

2306-7381

e-ISSN

2306-7381

Volume of the periodical

11

Issue of the periodical within the volume

12

Country of publishing house

CH - SWITZERLAND

Number of pages

13

Pages from-to

1-13

UT code for WoS article

001384508600001

EID of the result in the Scopus database

2-s2.0-85213476315