Optimizing the conventional method of sperm freezing in liquid nitrogen vapour for Wallachian sheep conservation program
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F21%3A88409" target="_blank" >RIV/60460709:41210/21:88409 - isvavai.cz</a>
Result on the web
<a href="https://www.agriculturejournals.cz/web/cjas.htm?type=article&id=226_2020-CJAS" target="_blank" >https://www.agriculturejournals.cz/web/cjas.htm?type=article&id=226_2020-CJAS</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/226/2020-CJAS" target="_blank" >10.17221/226/2020-CJAS</a>
Alternative languages
Result language
angličtina
Original language name
Optimizing the conventional method of sperm freezing in liquid nitrogen vapour for Wallachian sheep conservation program
Original language description
The aim of the present study was to optimize the conventional method of sperm freezing in liquid nitrogen LN2 vapour for successful cryopreservation of Wallachian ram sperm, the genetic resources of the Czech Republic. Sperm in straws were frozen using the conventional freezing method via a static exposure of sperm doses to LN2 vapour, or by four different modified freezing methods. Under modified freezing, straws were frozen by a discontinuous, time-dependent decremental change in the distance between the straws and the surface of LN 2. The viability of sperm was evaluated by flow cytometry after sperm equilibration, and immediately after thawing. Besides the observed inter-sire and daily variation, the obtained results suggest the methodological weakness of the conventional freezing method via the static exposure of sperm doses to LN 2 vapour. With the use of the optimized freezing procedure, all parameters of thawed sperm were significantly improved in comparison with the conventio
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40101 - Agriculture
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Czech Journal of Animal Science
ISSN
1212-1819
e-ISSN
1805-9309
Volume of the periodical
66
Issue of the periodical within the volume
2
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
10
Pages from-to
55-64
UT code for WoS article
000620367500003
EID of the result in the Scopus database
2-s2.0-85102839144