Oryzalin-Induced polyploidy enhances natural metabolite accumulation and functional bioactivities in novel Callisia fragrans genotype

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F25%3A102090" target="_blank" >RIV/60460709:41210/25:102090 - isvavai.cz</a>

Alternative codes found

RIV/60460709:41340/25:102090

Result on the web

<a href="https://www.nature.com/articles/s41598-025-10588-9" target="_blank" >https://www.nature.com/articles/s41598-025-10588-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-025-10588-9" target="_blank" >10.1038/s41598-025-10588-9</a>

Result language

angličtina

Original language name

Oryzalin-Induced polyploidy enhances natural metabolite accumulation and functional bioactivities in novel Callisia fragrans genotype

Original language description

The reorientation of Callisia fragrans (Lindl.) Woodson, from therapeutic to ornamental use, exemplifies a broader domestication trend, favoring aesthetics over medicinal properties. Renewed phytomedicinal interest and the rise of plant extract markets drive demand for organic, sustainable consumer products, heightening the competition for superior cultivars. Synthetic polyploidization using oryzalin was conducted to obtain high-quality cultivars of C. fragrans, facilitating enhanced phenotypic and biological traits without genetic modification. This study aimed to explore the metabolic spectrum and biological activities of oryzalin-induced polyploid C. fragrans for its advanced medicinal application. Flow cytometric analysis confirmed the ploidy level of the plants. Consequently, GC-FID and 1H NMR analyses revealed distinct metabolite profiles, with increased ethyl stearate, malic acid, gallic acid, fumaric acid, and unique compounds like (Z)-11-eicosenoic acid and dodecan-1-ol in polyploids. Polyploid extracts demonstrated exceptional antioxidant capacity, with DPPH, ORAC, and ABTS assays showing higher radical scavenging and oxygen absorbance abilities than diploid extracts. The polyploid extract showed enhanced antimicrobial activity against skin pathogens, including Methicillin-resistant Staphylococcus aureus (MRSA). Callisia extracts, meticulously at a low concentration of 25 mu g/mL, showed cytoprotective effects on HT-29 cells, mitigating H2O2-induced oxidative stress. Furthermore, treatment with polyploid extract was associated with the downregulation of the expression of pro-inflammatory enzymes COX-1 and COX-2, suggesting a potentially greater anti-inflammatory effect compared to the diploid extract. These findings depict enhanced metabolite accumulation and biological activities in polyploid compared than diploid progenitor, highlighting the potential of the novel polyploid C. fragrans variety for future therapeutic applications, particularly in pharmaceutical and cosmetic industries.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10700 - Other natural sciences

Project

<a href="/en/project/QK1910209" target="_blank" >QK1910209: Research and development of the medicinal mushroom production in the Czech Republic and their innovative application in functional foods</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Reports

ISSN

2045-2322

e-ISSN

2045-2322

Volume of the periodical

15

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

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UT code for WoS article

001530067200018

EID of the result in the Scopus database

2-s2.0-105010748496