Mutual interaction of silymarin flavonolignans with human gut microbiota

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F25%3A102222" target="_blank" >RIV/60460709:41210/25:102222 - isvavai.cz</a>

Result on the web

<a href="https://amsacta.unibo.it/id/eprint/8570/3/Book%20of%20Proceedings%201st%20INFOGUT%20ANNUAL%20MEETING%20complete%2024%2010%202025.pdf" target="_blank" >https://amsacta.unibo.it/id/eprint/8570/3/Book%20of%20Proceedings%201st%20INFOGUT%20ANNUAL%20MEETING%20complete%2024%2010%202025.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Mutual interaction of silymarin flavonolignans with human gut microbiota

Original language description

Introduction. Silymarin, an extract from fruits of the milk thistle Silybum marianum (L.) Gaertn., is made up of the flavonolignans silybin A and B, isosilybin A and B, silychristin A and B, silydianin and the flavanol taxifolin. Minor components include isosilychristin, 2,3- dehydrosilybin, 2,3-dehydrosilychristin and 2,3-dehydrosilydianin. The bioavailability of silymarin components is 20–50% due to low. The aim of the study was to evaluate the complex interactions of silymarin and its components with gut microbiota both ex vivo and in vivo. Materials and Methods. Biotransformation of silymarin components was studied ex vivo, using batch incubations inoculated by fecal slurry and HPLC/MS. The interaction of the silymarin complex was investigated with a focus on aging populations. Using advanced techniques such as NGS, NMR and LC-MS, we analyzed the dual impact of the microbiome on silymarin metabolism and the effect of silymarin on the microbiome’s structure and function. Finally, in 33 healthy adult male volunteers, who received 200 mg of silymarin orally twice daily for three months, formation of metabolites in urine and feces was evaluated by HPLC/MS and bacterial composition of feces was investigated by NGS. Results and Discussion. At 200 mg/L the flavonolignans were resistant to the metabolic action of microbiota. At 10 mg/L, biotransformation of flavonolignans was much slower than that of taxifolin. Silybin, isosilybin, and 2,3-dehydrosilybin underwent mostly demethylation, silychristin was predominantly reduced. Silydianin, 2,3-dehydrosilychristin and 2,3- dehydrosilydianin were reduced and decarbonylation and cysteine conjugation proceeded. No low-molecular-weight phenolic metabolites were detected. Silymarin significantly altered the metabolism of the gut microbiota, decreasing short-chain fatty acid production and glucose utilization. Healthy elders (70–80 years) showed a significant increase in a specific catabolite associated with Oscillibacter. Conversely, healthy young donors (12–45 years) exhibited faster breakdown of silymarin components, particularly isosilybin B, which negatively correlated with higher abundance of Faecalibacterium and Erysipelotrochaceae UCG-003. In volunteers, a correlation was found between the number of metabolites and the composition of the intestinal microbiota. Conclusion. Silymarin interacts with the microbiome, influences its composition and metabolic profile. The amount of metabolites in feces depend on the composition of the gut microbiota that is responsible for large inter-individual differences

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

30308 - Nutrition, Dietetics

Project

<a href="/en/project/GA23-04655S" target="_blank" >GA23-04655S: Role of prenylation and glycosylation patterns in anti-inflammatory activity and metabolism of natural phenolic compounds</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Book of Conference Proceedings 1 st INFOGUT Annual Meeting

ISBN

9788854972100

ISSN

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e-ISSN

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Number of pages

2

Pages from-to

16-17

Publisher name

University of Bologna

Place of publication

Bologna

Event location

Bologna

Event date

Jan 1, 2025

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

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