Isolation of The Probiotic Strains from Honeybees and Assessment of Their Effect on Honeybee Health

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F25%3A102225" target="_blank" >RIV/60460709:41210/25:102225 - isvavai.cz</a>

Result on the web

<a href="https://heyzine.com/flip-book/18e8f2d7eb.html#page/4" target="_blank" >https://heyzine.com/flip-book/18e8f2d7eb.html#page/4</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Isolation of The Probiotic Strains from Honeybees and Assessment of Their Effect on Honeybee Health

Original language description

Honeybees harbor a core microbiota in their digestive tract, including species of lactobacilli and bifidobacteria, which play a crucial role in maintaining host health and defending against harmful pathogens. One key function of these bacteria is their ability to inhibit the growth of bacterial pathogens. This study assessed the inhibitory properties of various isolates from the honeybee gut against five strains of honeybee pathogens, providing a foundation for selecting strains that may enhance the bees’ natural defense. In this study, 111 bacterial strains (62 lactobacilli and 49 bifidobacteria) were isolated from the digestive tracts of honeybees collected from eight different sites in the Czech Republic. These strains were identified using 16S rRNA gene sequencing and subsequently tested for their ability to inhibit four clinical isolates of Paenibacillus larvae, one clinical isolate of Melissococcus plutonius, and one of the opportunistic pathogen Serratia marcescens by the plate agar diffusion method. The results showed that 29 strains (15 bifidobacteria and 14 lactobacilli) were able to effectively inhibit at least two clinical isolates of P. larvae, with inhibition zones ? 21 mm and representing approximately 26% of all strains tested. Additionally, the inhibition of M. plutonius and S. marcescens was observed in three and 20 strains, respectively. The most significant inhibitory effects were found in species Bifidobacterium asteroides, B. choladohabitans, B. polysaccharolyticum, Bifidobacterium sp., Lactobacillus apis, L. helsingborgensis, L. kullabergensis, and L. melliventris. These findings emphasize that the probiotic potential of lactobacilli and bifidobacteria varies by strain. Some strains exhibited stronger inhibitory effects against multiple clinical P. larvae isolates, underscoring their promise as potential probiotics for honeybee health. Due to the variation in inhibitory activity among strains, selecting those with proven broad-spectrum antimicrobial effects against relevant honeybee pathogens is crucial. This selection forms the basis for using specific probiotic strains as a strategy to mitigate bacterial diseases in honeybee populations, ultimately supporting colony health and resilience.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

40101 - Agriculture

Project

<a href="/en/project/QK21010088" target="_blank" >QK21010088: Development of a feed supplement for honey bee immunity support, together with a production technology and ulitisation of its side products as food</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů