Effects of Defined Pig Microbiota on Acute Salmonellosis in Gnotobiotic Piglets
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F25%3A106438" target="_blank" >RIV/60460709:41210/25:106438 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/25:10515647
Result on the web
<a href="https://faseb.onlinelibrary.wiley.com/doi/10.1096/fj.202503234R" target="_blank" >https://faseb.onlinelibrary.wiley.com/doi/10.1096/fj.202503234R</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1096/fj.202503234" target="_blank" >10.1096/fj.202503234</a>
Alternative languages
Result language
angličtina
Original language name
Effects of Defined Pig Microbiota on Acute Salmonellosis in Gnotobiotic Piglets
Original language description
Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) is a ubiquitous Salmonella serovar that causes enterocolitis in humans, livestock, poultry, and some pets. Gnotobiotic animals are suitable for studying the development of initial colonization in the gastrointestinal tract, host-microbiota cross-talk, and interactions among microbiota strains. To clarify the impact of pre-colonization of the germ-free piglets with defined pig microbiota (DPM) on the subsequent infection with S. Typhimurium LT2 (LT2), we focused on the acute host immune response. Colonization with DPM did not cause signs of enterocolitis (sleepiness, anorexia, fever, and diarrhea), histological changes in the intestine, the density of acid mucin-producing cells, expression of villin, claudin-1, occludin, TLR4, MD-2, CD14, LBP, MyD88, and TRIF, induction of inflammatory cytokines IL-1ß, IL-6, IL-8, IL-10, IL-12/23p40, TNF-?, IFN-?, and HMGB1. LT2 translocated into the mesenteric lymph nodes, liver, spleen, and blood of the LT2 group, as well as damaged the intestinal structure, and upregulated intestinal inflammatory cytokine levels. The pre-colonization with DPM ameliorated the LT2-induced signs of enterocolitis, intestinal damage, and inflammatory changes. Changes in intestinal levels of soluble TLR4 and TLR2 indicate the possible inclusion of soluble TLRs in the regulation of the inflammatory process. The controlled initial colonization of the newborn gastrointestinal tract with a defined microbial consortium can increase newborn resistance to enteric pathogens and promote thriving of young animals.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
Result continuities
Project
<a href="/en/project/GA21-15621S" target="_blank" >GA21-15621S: Defined minimal microbiota in protection against foodborne pathogen Salmonella enterica</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
FASEB JOURNAL
ISSN
0892-6638
e-ISSN
0892-6638
Volume of the periodical
40
Issue of the periodical within the volume
1
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
17
Pages from-to
1-17
UT code for WoS article
001651972700001
EID of the result in the Scopus database
2-s2.0-105026349833