The Biological Effects of Complete Gasoline Engine Emissions Exposure in a 3D Human Airway Model (MucilAir(TM)) and in Human Bronchial Epithelial Cells (BEAS-2B)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41310%2F19%3A81925" target="_blank" >RIV/60460709:41310/19:81925 - isvavai.cz</a>
Alternative codes found
RIV/68378041:_____/19:00518531 RIV/68407700:21220/19:00335128 RIV/68407700:21230/19:00335128 RIV/00216208:11310/19:10409254
Result on the web
<a href="https://www.mdpi.com/1422-0067/20/22/5710" target="_blank" >https://www.mdpi.com/1422-0067/20/22/5710</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms20225710" target="_blank" >10.3390/ijms20225710</a>
Alternative languages
Result language
angličtina
Original language name
The Biological Effects of Complete Gasoline Engine Emissions Exposure in a 3D Human Airway Model (MucilAir(TM)) and in Human Bronchial Epithelial Cells (BEAS-2B)
Original language description
The biological effects induced by complete engine emissions in a 3D model of the human airway (MucilAir(TM)) and in human bronchial epithelial cells (BEAS-2B) grown at the air-liquid interface were compared. The cells were exposed for one or five days to emissions generated by a Euro 5 direct injection spark ignition engine. The general condition of the cells was assessed by the measurement of transepithelial electrical resistance and mucin production. The cytotoxic effects were evaluated by adenylate kinase (AK) and lactate dehydrogenase (LDH) activity. Phosphorylation of histone H2AX was used to detect double-stranded DNA breaks. The expression of the selected 370 relevant genes was analyzed using next-generation sequencing. The exposure had minimal effects on integrity and AK leakage in both cell models. LDH activity and mucin production in BEAS-2B cells significantly increased after longer exposures DNA breaks were also detected. The exposure affected CYP1A1 and HSPA5 expression in MucilAir(TM).
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Molecular Sciences
ISSN
1422-0067
e-ISSN
1422-0067
Volume of the periodical
2019
Issue of the periodical within the volume
20
Country of publishing house
CH - SWITZERLAND
Number of pages
22
Pages from-to
1-22
UT code for WoS article
000502786800192
EID of the result in the Scopus database
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