The biological effects of complete gasoline engine emissions exposure in a 3D human airway model (Mucilairtm) and in human bronchial epithelial cells (BEAS-2B)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F19%3A00518531" target="_blank" >RIV/68378041:_____/19:00518531 - isvavai.cz</a>

Alternative codes found

RIV/68407700:21220/19:00335128 RIV/68407700:21230/19:00335128 RIV/00216208:11310/19:10409254 RIV/60460709:41310/19:81925

Result on the web

<a href="https://www.mdpi.com/1422-0067/20/22/5710" target="_blank" >https://www.mdpi.com/1422-0067/20/22/5710</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ijms20225710" target="_blank" >10.3390/ijms20225710</a>

Result language

angličtina

Original language name

The biological effects of complete gasoline engine emissions exposure in a 3D human airway model (Mucilairtm) and in human bronchial epithelial cells (BEAS-2B)

Original language description

The biological effects induced by complete engine emissions in a 3D model of the human airway (MucilAir™) and in human bronchial epithelial cells (BEAS-2B) grown at the air–liquid interface were compared. The cells were exposed for one or five days to emissions generated by a Euro 5 direct injection spark ignition engine. The general condition of the cells was assessed by the measurement of transepithelial electrical resistance and mucin production. The cytotoxic effects were evaluated by adenylate kinase (AK) and lactate dehydrogenase (LDH) activity. Phosphorylation of histone H2AX was used to detect double-stranded DNA breaks. The expression of the selected 370 relevant genes was analyzed using next-generation sequencing. The exposure had minimal effects on integrity and AK leakage in both cell models. LDH activity and mucin production in BEAS-2B cells significantly increased after longer exposures, DNA breaks were also detected. The exposure affected CYP1A1 and HSPA5 expression in MucilAir™. There were no effects of this kind observed in BEAS-2B cells, in this system gene expression was rather affected by the time of treatment. The type of cell model was the most important factor modulating gene expression. In summary, the biological effects of complete emissions exposure were weak. In the specific conditions used in this study, the effects observed in BEAS-2B cells were induced by the exposure protocol rather than by emissions and thus this cell line seems to be less suitable for analyses of longer treatment than the 3D model.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30108 - Toxicology

Project

<a href="/en/project/GA18-04719S" target="_blank" >GA18-04719S: Mechanisms of toxicity of gasoline engine emissions in 3D tissue cultures and a model bronchial epithelial cell line</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Molecular Sciences

ISSN

1422-0067

e-ISSN

—

Volume of the periodical

20

Issue of the periodical within the volume

22

Country of publishing house

CH - SWITZERLAND

Number of pages

22

Pages from-to

5710

UT code for WoS article

000502786800192

EID of the result in the Scopus database

2-s2.0-85075115173