Phytochemical Screening and GC-MS Analysis of Methanolic and Aqueous Extracts of Ocimum kilimandscharicum Leaves
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41320%2F23%3A97198" target="_blank" >RIV/60460709:41320/23:97198 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1021/acsomega.3c05554" target="_blank" >http://dx.doi.org/10.1021/acsomega.3c05554</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acsomega.3c05554" target="_blank" >10.1021/acsomega.3c05554</a>
Alternative languages
Result language
angličtina
Original language name
Phytochemical Screening and GC-MS Analysis of Methanolic and Aqueous Extracts of Ocimum kilimandscharicum Leaves
Original language description
Kenyans have long utilized Ocimum kilimandscharicum, an East African permanent evergreen plant, to treat measles, stomachaches, diarrhea, mosquito bites (anti-insect), congested chest, cough, and colds. Using conventional qualitative and quantitative techniques, this study was done to identify the secondary metabolites in O. kilimandscharicum leaf extracts. The chemical content of the crude extracts from the leaves of O. kilimandscharicum has also been investigated and characterized using gas chromatography-mass spectrometry (GC-MS). By using a 1:20 dilution in methanol, in cold maceration, a fine powder of O. kilimandscharicum was first extracted then filtered and concentrated after 72 h utilizing a rotary evaporator. By using also a 1:20 dilution in water at 80 degrees C, a fine powder of O. kilimandscharicum was extracted and then filtrated and lyophilized 1 h later. Each extract underwent further gas chromatography-mass spectrometry testing. We found that both extracts contain secondary metabolites such as alkaloids, phenolics, flavonoids, saponins, and tannins. However, the overall amount of phytochemicals in each solvent varied significantly. Total phenolics contents (TPCs) were 5.6 +/- 1.20 and 10.8 +/- 1.00 mg, total flavonoid contents (TFCs) were 8.2 +/- 0.4 and 39.6 +/- 2.2 mg, total tannin contents (TTCs) were 0 +/- 0.00 and 10.5 +/- 0.4 mg, the total alkaloid content (TAC) was 49.2 +/- 0.40%, and the total saponin content (TSC) was 38 +/- 2.00%. Additionally the gas chromatography-mass spectrometry, revealed a number of high- and low-molecular-weight bioactive molecules at various concentrations for each extract. We also found an inhibitory effect on adhP and chbR gene expression of Staphylococcus aureus and Salmonella choleraesuius, respectively. Hence, these chemicals could potentially have a biological and pharmacological significance. Therefore, the discovery of many physiologically active chemicals in the leaf extracts of O. kilimandscharicum justifies future biological and pharmaceutical research. (TR Abstract Type: graphical)(TR Abstract Type: unknown)
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ACS Omega
ISSN
2470-1343
e-ISSN
2470-1343
Volume of the periodical
8
Issue of the periodical within the volume
50
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
47560-47572
UT code for WoS article
001128039300001
EID of the result in the Scopus database
2-s2.0-85180122149