Exploring melatonin’s influence on secondary metabolism of Echinacea purpurea callus cultures
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41340%2F25%3A103776" target="_blank" >RIV/60460709:41340/25:103776 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/article/10.1007/s11240-025-03034-1#Sec1" target="_blank" >https://link.springer.com/article/10.1007/s11240-025-03034-1#Sec1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11240-025-03034-1" target="_blank" >10.1007/s11240-025-03034-1</a>
Alternative languages
Result language
angličtina
Original language name
Exploring melatonin’s influence on secondary metabolism of Echinacea purpurea callus cultures
Original language description
Echinacea purpurea, a key medicinal herb of the Asteraceae family, is rich in antioxidants and anti-inflammatory compounds, making it valuable in pharmaceuticals. In vitro cultures enhance sustainable metabolite production, often stimulated by elicitors. This study employed TDZ to induce E. purpurea callus cultures and assessed the impact of melatonin on biomass, antioxidant potential, and secondary metabolites. The callus cultures treated with 25 µM melatonin showed the highest biomass (FW: 208.62 g/L, DW: 11.87 g/L), total phenolic (TPP: 132.8 mg GAE/L), and flavonoid (TFP: 585.9 mg QE/L) production. Antioxidant activity peaked at this concentration, with 92.7% DPPH scavenging and 63.2 µg AAE/mg total antioxidant capacity. Moreover, the callus cultures exhibited notable enzyme inhibitory effects, with 68% inhibition against pancreatic lipase, 22% inhibition against alpha-glucosidase, and 40% inhibition against alpha-amylase, following treatment with 50 µM, 25 µM, and 10 µM of melatonin, respectively. These findings further highlight that the current approach should be further investigated to explore innovative treatments for obesity and diabetes. UPLC-MS analysis confirmed enhanced metabolite synthesis at 25–50 µM melatonin, while amino acid production peaked at 1µM. These findings highlight melatonin elicitation as an effective strategy for improving biomass, phenolics, and bioactivity in E. purpurea callus cultures.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40101 - Agriculture
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Cell Tissue and Organ Culture
ISSN
0167-6857
e-ISSN
0167-6857
Volume of the periodical
161
Issue of the periodical within the volume
2
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
15
Pages from-to
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UT code for WoS article
001474353000001
EID of the result in the Scopus database
2-s2.0-105003321126