Exogenous applications of humic acid and salicylic acid differentially affect biomass accumulation and production of rare pharmacologically active withanolides in in vitro cultures of Withania coagulans
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41340%2F25%3A103790" target="_blank" >RIV/60460709:41340/25:103790 - isvavai.cz</a>
Result on the web
<a href="https://www.tandfonline.com/doi/full/10.1080/11263504.2025.2489989?scroll=top&needAccess=true#abstract" target="_blank" >https://www.tandfonline.com/doi/full/10.1080/11263504.2025.2489989?scroll=top&needAccess=true#abstract</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/11263504.2025.2489989" target="_blank" >10.1080/11263504.2025.2489989</a>
Alternative languages
Result language
angličtina
Original language name
Exogenous applications of humic acid and salicylic acid differentially affect biomass accumulation and production of rare pharmacologically active withanolides in in vitro cultures of Withania coagulans
Original language description
Ashwagandha (Withania coagulans) is a medicinal plant valued for its withanolide content, known for anti-cancer, anti-microbial, immunomodulatory, and hepatoprotective properties.However, due to habitat losss and overharvesting, it is now considered endangered. This study aimed to enhance biomass and withanolide production in W. coagulans callus cultures using humic acid (HA) and salicylic acid (SA) as elicitors. HA at 10 µM significantly boosted biomass (fresh weight (FW): 3.6 g/L, dry weight (DW): 267 mg/L), antioxidant enzymatic activity (peroxidase (POD) and superoxidase dismutase (SOD)) and non-enzymatic antioxidant potential, including DPPH (80.2%), ABTS (287.1 µM of Trolox equivalent antioxidant capacity (TEAC) and FRAP (548.2 µM TEAC). In contrast, SA at 60 µM negatively affected biomass and phytochemical content. HPLC analysis confirmed enhanced accumulation of withanolides and coagulanolide under HA treatment. Overall, low concentrations of HA and SA proved more effective and economically viable than high concentrations, enhancing both growth and secondary metabolite production. These findings support optimized in vitro elicitation strategies for conservation and sustainable use of W. coagulans, offering potential for commerical-scale cultivation and pharmaceutical applications. Future research may focus on understanding the molecular mechanisms behind elicitor responses and scaling up production systems to meet industrial demands.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
40101 - Agriculture
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLANT BIOSYSTEMS
ISSN
1126-3504
e-ISSN
1126-3504
Volume of the periodical
159
Issue of the periodical within the volume
3
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
15
Pages from-to
581-595
UT code for WoS article
001484049000001
EID of the result in the Scopus database
2-s2.0-105004464047