Immunochemical screening of isoflavones in the Rutaceae family

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41610%2F04%3A9841" target="_blank" >RIV/60460709:41610/04:9841 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Immunochemical screening of isoflavones in the Rutaceae family

Original language description

Isoflavones (3-phenyl chromones) are phytoalexins abundant in the Fabaceae, however their biosynthesis was described in over 20 other families, e.g. Rosaceae, Iridaceae, Asteraceae, Poaceae. Molecular biology data indicate occurrence of genes for isoflavonoid metabolic pathway even in higher number of families (1), although it is difficult to obtain the evidence of their biochemical activity. Recently, we have found isoflavonoids in the Rutaceae family (1). In our recent research, we combine immunochemical approaches and HPLC-MS. EXPERIMENTAL: Five immunoassays for early products of the isoflavonoid pathway (i.e. daidzein, formononetin, genistein and biochanin A and their glycosides) were adapted for phytochemical studies. Tested plant material (leavesfrom apical branches) was frozen immediately after collecting and kept in ?20 °C until lyophilized. A limited number of samples were divided to two aliquots of which one was dried in laboratory temperature in shadow. Dry samples were pul

Czech name

Immunochemický screening izoflavonů v čeledi Rutaceae

Czech description

Isoflavones (3-phenyl chromones) are phytoalexins abundant in the Fabaceae, however their biosynthesis was described in over 20 other families, e.g. Rosaceae, Iridaceae, Asteraceae, Poaceae. Molecular biology data indicate occurrence of genes for isoflavonoid metabolic pathway even in higher number of families (1), although it is difficult to obtain the evidence of their biochemical activity. Recently, we have found isoflavonoids in the Rutaceae family (1). In our recent research, we combine immunochemical approaches and HPLC-MS. EXPERIMENTAL: Five immunoassays for early products of the isoflavonoid pathway (i.e. daidzein, formononetin, genistein and biochanin A and their glycosides) were adapted for phytochemical studies. Tested plant material (leavesfrom apical branches) was frozen immediately after collecting and kept in ?20 °C until lyophilized. A limited number of samples were divided to two aliquots of which one was dried in laboratory temperature in shadow. Dry samples were pul

Type

D - Article in proceedings

CEP classification

GM - Food industry

OECD FORD branch

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Project

<a href="/en/project/GA525%2F03%2F0352" target="_blank" >GA525/03/0352: Isoflavonoids in the Myrtaceae and the Rutaceae families</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

19th Meeting of Czech and Slovak Societies for Biochemistry and Molecular Biology

ISBN

80-244-0882-1

ISSN

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e-ISSN

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Number of pages

2

Pages from-to

215-216

Publisher name

Univerzita Palackého

Place of publication

Olomouc, Czech Republic

Event location

Olomouc, Czech Republic

Event date

Aug 31, 2004

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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