Polyclonal antibodies for detection of flour mite Acarus siro

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A_____%2F04%3A01%3A_3621" target="_blank" >RIV/60460709:_____/04:01:_3621 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Polyclonal antibodies for detection of flour mite Acarus siro
Original language description
Acaroid mites are serious pests of stored products and households. They produce allergens and transmit mycotoxin-producing fungi. The infestation of stored food by mites strongly decreases food safety and endangers public health. There is urgent need for
Czech name
Polyclonal antibodies pro detekci moucného cerva Acarus siro
Czech description
Acaroid mites are serious pests of stored products and households. They produce allergens and transmit mycotoxin-producing fungi. The infestation of stored food by mites strongly decreases food safety and endangers public health. There is urgent need forrapid detection methods such as ELISA. The aim of our work was therefore to prepare and characterise antibodies for detection of the flour mite (Acarus siro). Two polyclonal antibodies (anti- A.siroK53 and anti-A.siroK54) were prepared against an antigenin the form of protein fraction from whole body homogenates of Acarus siro. Specificity and sensitivity of the prepared antibodies were evaluated. We did not observe any cross-reactivity with stored beetles, moths, other mite species, micro-fungi, therearing diet of mites (yeast and wheat diet) and extracts from wheat kernels. Cross-reactivity to Acarus gracilis was found out. This phenomenon is discussed in relation to the evolution position of this species. The obtained antibodies (an

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GM - Food industry
OECD FORD branch
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Project
<a href="/en/project/QF4071" target="_blank" >QF4071: Integrated method of detection and monitoring of pest contamination of stored-grain and cereal products by physical, chemical, immunochemical and molecular techniques.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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