Formation and Temperature Stability of G-Quadruplex Structures Studied by Electronic and Vibration Circular Dichroism Spectroscopy Combined with Ab Initio Calculations

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F08%3A00020406" target="_blank" >RIV/60461373:22310/08:00020406 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22340/08:00020765

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Formation and Temperature Stability of G-Quadruplex Structures Studied by Electronic and Vibration Circular Dichroism Spectroscopy Combined with Ab Initio Calculations

Original language description

Variations in the structure of d(GGGA)5 oligonucleotide in the presence of Li+, Na+, and K+ ions and its temperature stability were studied using electronic and vibrational circular dichroism, IR absorption, and ab initio calculations with the Becke 3-Lee-Yang-Parr functional at the 6-31G** level. The samples were characterized by nondenaturing gel electrophoresis. Oligonucleotide d(GGGA)5in the presence of Li+ forms a nonplanar single tetramer, with angles of 102° and 171° between neighboring guanine bases. This tetramer changes its geometry at temperatures >50°C, but does not form a quadruplex structure. In the presence of Na+, the d(GGGA)5 structure was optimized to almost planar tetramers with an angle of 177° between neighboring guanines. The spectral results suggest that it stacks into a quadruplex helical structure. This quadruplex structure decayed to a single tetramer at temperatures >60°C. The Hartree-Fock energies imply that d(GGGA)5 prefers to form complexes with Na+ rather

Czech name

Formation and Temperature Stability of G-Quadruplex Structures Studied by Electronic and Vibration Circular Dichroism Spectroscopy Combined with Ab Initio Calculations

Czech description

Variations in the structure of d(GGGA)5 oligonucleotide in the presence of Li+, Na+, and K+ ions and its temperature stability were studied using electronic and vibrational circular dichroism, IR absorption, and ab initio calculations with the Becke 3-Lee-Yang-Parr functional at the 6-31G** level. The samples were characterized by nondenaturing gel electrophoresis. Oligonucleotide d(GGGA)5in the presence of Li+ forms a nonplanar single tetramer, with angles of 102° and 171° between neighboring guanine bases. This tetramer changes its geometry at temperatures >50°C, but does not form a quadruplex structure. In the presence of Na+, the d(GGGA)5 structure was optimized to almost planar tetramers with an angle of 177° between neighboring guanines. The spectral results suggest that it stacks into a quadruplex helical structure. This quadruplex structure decayed to a single tetramer at temperatures >60°C. The Hartree-Fock energies imply that d(GGGA)5 prefers to form complexes with Na+ rather

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CC - Organic chemistry

OECD FORD branch

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Project

<a href="/en/project/OC%20135" target="_blank" >OC 135: Detailed structural studies of bio-inspired compounds using advanced vibrational circular dichroism methods</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biopolymers

ISSN

0006-3525

e-ISSN

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Volume of the periodical

89

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

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UT code for WoS article

000252572800006

EID of the result in the Scopus database

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