Is non-buffered DMEM solution a suitable medium for in vitro bioactivity tests?
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F14%3A43898023" target="_blank" >RIV/60461373:22310/14:43898023 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22330/14:43898023
Result on the web
<a href="http://dx.doi.org/10.1039/c4tb00187g" target="_blank" >http://dx.doi.org/10.1039/c4tb00187g</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/c4tb00187g" target="_blank" >10.1039/c4tb00187g</a>
Alternative languages
Result language
angličtina
Original language name
Is non-buffered DMEM solution a suitable medium for in vitro bioactivity tests?
Original language description
Several laboratories had tested bioactivity of the materials in commercially available solution DMEM (Dulbecco's Modified Eagle's Medium) that is normally used for cultivation of cell cultures. The objective of this work was to find out whether it is possible to replace TRIS-buffered SBF currently used for bioactivity tests with the non-buffered DMEM solution. To understand the role of the organic part of the DMEM solution in the process of crystallization, we have prepared non-buffered solution simulating only its inorganic part (identified as I-solution). It was found that under static-dynamic test conditions calcite (CaCO3) and the amorphous phase of calcium phosphate (ACP) formed on the surface of the glassceramic (45S5 bioactive glass based) scaffold exposed to both solutions. Additionally, halite (NaCl) formed at the beginning of exposure to DMEM. Hydroxyapatite phase was not detected on the surface in either non-buffered solution. Organic components contained in the DMEM solutio
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CA - Inorganic chemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/TE01020390" target="_blank" >TE01020390: Centre of the development of advanced metallic biomaterials for medical implants</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Materials Chemistry
ISSN
0959-9428
e-ISSN
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Volume of the periodical
2
Issue of the periodical within the volume
2014
Country of publishing house
GB - UNITED KINGDOM
Number of pages
9
Pages from-to
"5068;5076"
UT code for WoS article
000339861000013
EID of the result in the Scopus database
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