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Is non-buffered DMEM solution a suitable medium for in vitro bioactivity tests?

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388980%3A_____%2F14%3A00436223" target="_blank" >RIV/61388980:_____/14:00436223 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1039/c4tb00187g" target="_blank" >http://dx.doi.org/10.1039/c4tb00187g</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1039/c4tb00187g" target="_blank" >10.1039/c4tb00187g</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Is non-buffered DMEM solution a suitable medium for in vitro bioactivity tests?

  • Original language description

    Several laboratories had tested bioactivity of the materials in commercially available solution DMEM (Dulbecco's Modified Eagle's Medium) that is normally used for cultivation of cell cultures. The objective of this work was to find out whether it is possible to replace TRIS-buffered SBF currently used for bioactivity tests with the non-buffered DMEM solution. To understand the role of the organic part of the DMEM solution in the process of crystallization, we have prepared non-buffered solution simulating only its inorganic part (identified as I-solution). It was found that under static-dynamic test conditions calcite (CaCO3) and the amorphous phase of calcium phosphate (ACP) formed on the surface of the glass-ceramic (45S5 bioactive glass based) scaffold exposed to both solutions. Additionally, halite (NaCl) formed at the beginning of exposure to DMEM. Hydroxyapatite phase was not detected on the surface in either non-buffered solution. Organic components contained in the DMEM soluti

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CA - Inorganic chemistry

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Materials Chemistry B

  • ISSN

    2050-750X

  • e-ISSN

  • Volume of the periodical

    2

  • Issue of the periodical within the volume

    31

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    9

  • Pages from-to

    5068-5076

  • UT code for WoS article

    000339861000013

  • EID of the result in the Scopus database