Effect of polymer modification on the cytocompatibility of human and rat cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F17%3A43915138" target="_blank" >RIV/60461373:22310/17:43915138 - isvavai.cz</a>
Result on the web
<a href="https://waset.org/Publication/effect-of-the-polymer-modification-on-the-cytocompatibility-of-human-and-rat-cells/10007722" target="_blank" >https://waset.org/Publication/effect-of-the-polymer-modification-on-the-cytocompatibility-of-human-and-rat-cells/10007722</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Effect of polymer modification on the cytocompatibility of human and rat cells
Original language description
Tissue engineering includes use of a combination of cells, engineering and materials methods for the formation of new viable tissue for a medical purpose. Scaffolds represent important components for tissue engineering. For some applications, the great advantage of several polymeric materials is their cytocompatibility and susceptibility to biodegradation. Poly(L-lactic acid) (PLLA) is a fully biodegradable polymer which further degrades into H2O and CO2. The crucial factor for successful application of tissue replacements is the interaction of a graft with its surrounding tissue. This interaction involves cell adhesion, adsorption of extracellular molecules and support of cell proliferation. In this experiment, the effect of the surface modification of biodegradable polymer (performed by plasma treatment) on the various cell types was studied. The surface parameters and changes of the physicochemical properties of modified PLLA substrates were studied by different methods. Surface wettability was determined by goniometry, surface morphology and roughness study was performed with atomic force microscopy and chemical composition was determined using photoelectron spectroscopy. The physicochemical properties were studied in relation to cytocompatibility of human osteoblast (MG 63 cells), rat vascular smooth muscle cells (VSMC) and human stem cells (ASC) of the adipose tissue in vitro. A fluorescence microscopy was chosen to study and compare cell-material interaction. Important parameters of the cytocompatibility like adhesion, proliferation, viability, shape, spreading of the above-mentioned cells were evaluated. It was found that the modification leads to the change of the surface wettability depending on the time of modification. By use of a short time (tens of s) of modification the wettability can be reduced, use a longer time (hundreds of s) leads to increase of wettability. The surface morphology is significantly influenced by duration of modification, too. The plasma treatment involves the formation of the crystallites, whose number increases with increasing time of modification. On the basis of physicochemical properties evaluation, three types of the cells were cultivated on the selected samples. Cell-material interactions are strongly affected by material chemical structure and surface morphology. It was proved that the plasma treatment of PLLA has a positive effect on the adhesion, spreading, homogeneity of distribution and viability of all cultivated cells. This effect was even more apparent for the VSMCs and ASCs which homogeneously covered almost the whole surface of the substrate after 7 days of cultivation. The viability of these cells was high. (more than 98% for VSMCs, 89-96% for ASCs).
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
20501 - Materials engineering
Result continuities
Project
<a href="/en/project/GBP108%2F12%2FG108" target="_blank" >GBP108/12/G108: Preparation, modification and characterization of materials by radiation</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
International Journal of Materials and Metallurgical Engineering
ISBN
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ISSN
2010-3778
e-ISSN
neuvedeno
Number of pages
6
Pages from-to
120-125
Publisher name
World Academy of Science, Engineering and Technology (WASET)
Place of publication
Connecticut
Event location
Sydney
Event date
Jan 26, 2017
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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