Synthesis of magnetic microparticles for isolation of DNA from PCR products

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F17%3A43915256" target="_blank" >RIV/60461373:22310/17:43915256 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Synthesis of magnetic microparticles for isolation of DNA from PCR products
Original language description
Nowadays, analysis of DNA plays an important role in molecular biology and diagnostics (such as diagnosis of microbial infections, forensic analysis and molecular genetics). With the rapid development of analytical methods such as NGS (Next Generation Sequencing) and DNA microarray followed by their use in various research and clinical assays there is a need for techniques for fast isolation of DNA from tissue samples and PCR products. Currently, isolation on a solid phase is preferred due to easier manipulation, short separation times and lower cost. Presented work was focused on synthesis of superparamagnetic nanoparticles (Fe3O4), their encapsulation by silica shell and further functionalization with amino groups. Different precursors, solvents and their ratios were used for synthesis of silica shell to obtain layers of various thickness. Those particles were then used for DNA isolation from PCR products. Optimization of the isolation was carried out and subsequently the influence of magnetic particles synthesis on the DNA isolation was studied.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Proceedings of the 5th International Conference on Chemical Technology
ISBN
978-80-86238-65-4
ISSN
2336-811X
e-ISSN
neuvedeno
Number of pages
6
Pages from-to
292-297
Publisher name
Česká společnost průmyslové chemie (ČSPCH)
Place of publication
Praha
Event location
Mikulov
Event date
Apr 10, 2017
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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