Coating of magnetic nanoparticles by silica layer and further functionalization for isolation of DNA from PCR products

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F18%3A43917597" target="_blank" >RIV/60461373:22310/18:43917597 - isvavai.cz</a>
Result on the web
<a href="https://www.icct.cz/AngiologyKlon-ICCT/media/system/2018/ICCT-2018-Proceedings.pdf" target="_blank" >https://www.icct.cz/AngiologyKlon-ICCT/media/system/2018/ICCT-2018-Proceedings.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Coating of magnetic nanoparticles by silica layer and further functionalization for isolation of DNA from PCR products
Original language description
Nowadays, analysis of DNA plays an important role in molecular biology and diagnostics (such as diagnosis of microbial infections, forensic analysis and molecular genetics). With the rapid development of analytical methods such as NGS (Next Generation Sequencing) and DNA microarray followed by their use in various research and clinical assays there is a need for techniques for fast isolation of DNA from tissue samples and PCR products. Currently, isolation on a solid phase is preferred due to easier manipulation, short separation times and lower cost. Presented work was focused on synthesis of superparamagnetic nanoparticles (Fe3O4), their encapsulation by silica shell and further functionalization with amino groups. Different precursors, solvents and their ratios were used for synthesis of silica shell to obtain layers of various thickness. Those particles were then used for DNA isolation from PCR products. Optimization of the isolation was carried out and subsequently the influence of magnetic particles synthesis on the DNA isolation was studied.
Czech name
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Czech description
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Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
PROCEEDINGS of the 6th International Conference on Chemical Technology
ISBN
978-80-86238-77-7
ISSN
2336-8128
e-ISSN
neuvedeno
Number of pages
6
Pages from-to
369-374
Publisher name
Česká společnost průmyslové chemie (ČSPCH)
Place of publication
Praha
Event location
Mikulov
Event date
Apr 16, 2018
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000461293800068

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