Reversible Lectin Binding to Glycan-Functionalized Graphene
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F21%3A43921944" target="_blank" >RIV/60461373:22310/21:43921944 - isvavai.cz</a>
Alternative codes found
RIV/68407700:21460/21:00356798
Result on the web
<a href="https://www.mdpi.com/1422-0067/22/13/6661/pdf" target="_blank" >https://www.mdpi.com/1422-0067/22/13/6661/pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms22136661" target="_blank" >10.3390/ijms22136661</a>
Alternative languages
Result language
angličtina
Original language name
Reversible Lectin Binding to Glycan-Functionalized Graphene
Original language description
The monolayer character of two-dimensional materials predestines them for application as active layers of sensors. However, their inherent high sensitivity is always accompanied by a low selectivity. Chemical functionalization of two-dimensional materials has emerged as a promising way to overcome the selectivity issues. Here, we demonstrate efficient graphene functionalization with carbohydrate ligands—chitooligomers, which bind proteins of the lectin family with high selectivity. Successful grafting of a chitooligomer library was thoroughly characterized, and glycan binding to wheat germ agglutinin was studied by a series of methods. The results demonstrate that the protein quaternary structure remains intact after binding to the functionalized graphene, and that the lectin can be liberated from the surface by the addition of a binding competitor. The chemoenzymatic assay with a horseradish peroxidase conjugate also confirmed the intact catalytic properties of the enzyme. The present approach thus paves the way towards graphene-based sensors for carbohydrate–lectin binding.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
—
Continuities
N - Vyzkumna aktivita podporovana z neverejnych zdroju
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Molecular Sciences
ISSN
1661-6596
e-ISSN
—
Volume of the periodical
22
Issue of the periodical within the volume
13
Country of publishing house
CH - SWITZERLAND
Number of pages
9
Pages from-to
6661
UT code for WoS article
000671186500001
EID of the result in the Scopus database
2-s2.0-85108207108