Reversible Lectin Binding to Glycan-Functionalized Graphene

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00551978" target="_blank" >RIV/61388971:_____/21:00551978 - isvavai.cz</a>
Alternative codes found
RIV/61388955:_____/21:00543633 RIV/61388963:_____/21:00543633
Result on the web
<a href="https://www.mdpi.com/1422-0067/22/13/6661" target="_blank" >https://www.mdpi.com/1422-0067/22/13/6661</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms22136661" target="_blank" >10.3390/ijms22136661</a>

Result language
angličtina
Original language name
Reversible Lectin Binding to Glycan-Functionalized Graphene
Original language description
The monolayer character of two-dimensional materials predestines them for application as active layers of sensors. However, their inherent high sensitivity is always accompanied by a low selectivity. Chemical functionalization of two-dimensional materials has emerged as a promising way to overcome the selectivity issues. Here, we demonstrate efficient graphene functionalization with carbohydrate ligands-chitooligomers, which bind proteins of the lectin family with high selectivity. Successful grafting of a chitooligomer library was thoroughly characterized, and glycan binding to wheat germ agglutinin was studied by a series of methods. The results demonstrate that the protein quaternary structure remains intact after binding to the functionalized graphene, and that the lectin can be liberated from the surface by the addition of a binding competitor. The chemoenzymatic assay with a horseradish peroxidase conjugate also confirmed the intact catalytic properties of the enzyme. The present approach thus paves the way towards graphene-based sensors for carbohydrate-lectin binding.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
21001 - Nano-materials (production and properties)

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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