The development of a butyrylcholinesterase porous pellet for innovative detection of cholinesterase inhibitors
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22320%2F17%3A43913512" target="_blank" >RIV/60461373:22320/17:43913512 - isvavai.cz</a>
Alternative codes found
RIV/62157124:16370/17:43875604
Result on the web
<a href="http://dx.doi.org/10.1016/j.ejps.2017.09.015" target="_blank" >http://dx.doi.org/10.1016/j.ejps.2017.09.015</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ejps.2017.09.015" target="_blank" >10.1016/j.ejps.2017.09.015</a>
Alternative languages
Result language
angličtina
Original language name
The development of a butyrylcholinesterase porous pellet for innovative detection of cholinesterase inhibitors
Original language description
The aim of the presented research was the preparation of an innovative carrier with significantly improved properties for the fast and sensitive detection of cholinesterase inhibitors such as nerve agents. This innovative carrier was in the form of spherical pellets containing different amounts of Neusilin. Neusilin is a synthetic and amorphous form of magnesium aluminometasilicate with a high specific surface area, and the immobilized enzyme butyrylcholinesterase with an activity of 50 nkat.g(-1). Pellets were prepared by the extrusion-spheronization method and dried in a hot air oven under two conditions - at 30 degrees C for 72 h and at 60 degrees C for 24 h. Dried pellets were consequently impregnated with a solution containing butyrylcholinesterase. Impregnated pellets were evaluated for their quality parameters, enzymatic activity and inhibition. Activity and inhibition were tested according to the standard Ellman's method. It was observed that the addition of Neusilin significantly increased the hardness, intraparticular porosity, sphericity and activity of the carriers as well as intensity of the color transition. Therefore it is shown that these carriers have unquestionable advantages over common carriers of their kind. Drying temperatures have been shown to have no effect on properties of pellets except for a change in their size. Results were confirmed by statistical evaluation using ANOVA and PCA.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30100 - Basic medicine
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
European Journal of Pharmaceutical Sciences
ISSN
0928-0987
e-ISSN
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Volume of the periodical
109
Issue of the periodical within the volume
2017
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
548-555
UT code for WoS article
000413325000057
EID of the result in the Scopus database
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