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The development of a butyrylcholinesterase porous pellet for innovative detection of cholinesterase inhibitors

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22320%2F17%3A43913512" target="_blank" >RIV/60461373:22320/17:43913512 - isvavai.cz</a>

  • Alternative codes found

    RIV/62157124:16370/17:43875604

  • Result on the web

    <a href="http://dx.doi.org/10.1016/j.ejps.2017.09.015" target="_blank" >http://dx.doi.org/10.1016/j.ejps.2017.09.015</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.ejps.2017.09.015" target="_blank" >10.1016/j.ejps.2017.09.015</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    The development of a butyrylcholinesterase porous pellet for innovative detection of cholinesterase inhibitors

  • Original language description

    The aim of the presented research was the preparation of an innovative carrier with significantly improved properties for the fast and sensitive detection of cholinesterase inhibitors such as nerve agents. This innovative carrier was in the form of spherical pellets containing different amounts of Neusilin. Neusilin is a synthetic and amorphous form of magnesium aluminometasilicate with a high specific surface area, and the immobilized enzyme butyrylcholinesterase with an activity of 50 nkat.g(-1). Pellets were prepared by the extrusion-spheronization method and dried in a hot air oven under two conditions - at 30 degrees C for 72 h and at 60 degrees C for 24 h. Dried pellets were consequently impregnated with a solution containing butyrylcholinesterase. Impregnated pellets were evaluated for their quality parameters, enzymatic activity and inhibition. Activity and inhibition were tested according to the standard Ellman&apos;s method. It was observed that the addition of Neusilin significantly increased the hardness, intraparticular porosity, sphericity and activity of the carriers as well as intensity of the color transition. Therefore it is shown that these carriers have unquestionable advantages over common carriers of their kind. Drying temperatures have been shown to have no effect on properties of pellets except for a change in their size. Results were confirmed by statistical evaluation using ANOVA and PCA.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30100 - Basic medicine

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2017

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    European Journal of Pharmaceutical Sciences

  • ISSN

    0928-0987

  • e-ISSN

  • Volume of the periodical

    109

  • Issue of the periodical within the volume

    2017

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    8

  • Pages from-to

    548-555

  • UT code for WoS article

    000413325000057

  • EID of the result in the Scopus database