Mechanisms of Anammox Adaptation to High Temperatures: Increased Cyclization of Ladderane Lipids and Proteomic Insights
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22320%2F25%3A43930500" target="_blank" >RIV/60461373:22320/25:43930500 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22330/25:43930500
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S2213343725003239" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2213343725003239</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jece.2025.115628" target="_blank" >10.1016/j.jece.2025.115628</a>
Alternative languages
Result language
angličtina
Original language name
Mechanisms of Anammox Adaptation to High Temperatures: Increased Cyclization of Ladderane Lipids and Proteomic Insights
Original language description
Although anammox-based processes have been widely applied in mesophilic conditions of reject water and recently in mainstream conditions, the potential of their implementation in high-temperature wastewaters remains largely unexplored. Therefore, this study investigated the operation parameters for the successful adaptation of anammox bacteria and the mechanisms involved at the proteomic and cellular levels, including unique ladderane lipids. For this purpose, ‘Candidatus Brocadia’ enrichment was cultivated in two fed-batch reactors (FBRs) at a lab scale. The temperature of one FBR was gradually increased from 30 to 40 °C while the other FBR was maintained at 30 °C with four consecutive replicates of this experiment. To ensure successful adaptation, the original loading rate needed to be reduced by at least half, or preferably kept below 50 % of the specific anammox activity level at that time. The most notable adaptation mechanisms included: (1) upregulation of chaperones and (2) doubled ladderane cyclization via the replacement of nonladderane fatty acid by a ladderane fatty acid in ladderane lipids (p-value 0.005). To the best of our knowledge, this is the first study to describe the novel mechanism of ladderane cyclization, which, together with other adaptation strategies, presents crucial indicators in anammox adaptation to high-temperature wastewaters.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20801 - Environmental biotechnology
Result continuities
Project
<a href="/en/project/GA20-13766S" target="_blank" >GA20-13766S: Unique ladderane phospholipids of anammox bacteria: potential valuable resource from sewage</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Environmental Chemical Engineering
ISSN
2213-2929
e-ISSN
2213-3437
Volume of the periodical
13
Issue of the periodical within the volume
2
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
10
Pages from-to
115628-115637
UT code for WoS article
001434268800001
EID of the result in the Scopus database
2-s2.0-85216659765