Correlation study of enzyme-linked immunosorbent assay and high-performance liquid chromatography/tandem mass spectrometry method for the determination of N-methylcarbamate insecticides in baby food
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F03%3A00008477" target="_blank" >RIV/60461373:22330/03:00008477 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Correlation study of enzyme-linked immunosorbent assay and high-performance liquid chromatography/tandem mass spectrometry method for the determination of N-methylcarbamate insecticides in baby food
Original language description
In this work, a correlation study of monoclonal antibody-based enzyme-linked immunosorbent assays (ELISAs) and a liquid chromatography-electrospray mass spectrometric (HPLC/ESI/MS/MS) method for the determination of N-methyl carbamate insecticides carbofuran, carbaryl and methiocarb in fruit baby food is presented. The comparison of performance characteristics of the two methods was carried out by simultaneous analysis of apple-strawberry baby food (GPC purified) extracts spiked with N-methylcarbamatesat six different concentration levels. Results obtained by ELISA correlated well with those obtained by LC/MS/MS, both in terms of trueness and precision. Recoveries, i.e. the ratio of the determined concentration to the known spiked concentration, werein the 60-100% range for ELISA and in the 73-104 % range by LC/MS/MS with the RSDs from 7 replicate analyses 3.6 - 23.3 % and 1.7-8.2 %, respectively. The influence of sample pre-treatment on the analytical performance of immunoassay meth
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Anal.Chim.Acta
ISSN
0003-2670
e-ISSN
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Volume of the periodical
495
Issue of the periodical within the volume
1-2
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
10
Pages from-to
123-132
UT code for WoS article
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EID of the result in the Scopus database
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