Optimisation of enzyme-linked immunosorbent assay for the detection of Listeria monocytogenes

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F03%3A00008921" target="_blank" >RIV/60461373:22330/03:00008921 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Optimisation of enzyme-linked immunosorbent assay for the detection of Listeria monocytogenes
Original language description
Listeria monocytogenes is a gram-positive rod-shaped bacterium associated with a variety of environments including soils, water, sewage, silage, as well as plant and animal food products. Although reported cases of human foodborne listeriosis are rare, the incidence of serious illness and death in affected individuals is high. The detection of Listeria monocytogenes cells by conventional microbiological methods takes 5-6 days. We present here optimizations of a rapid, reliable and specific antibody-based assay. A model system consists of an enrichment stage and end-point detection by enzyme linked immunosorbent assay. Listeria cells were cultivated in selective medium in first step. Enrichment stage was followed by direct sandwich format of ELISA. It was found both convenient culture medium for cultivation of bacterial cells for use in the ELISA and optimal buffers for a whole method. From all tested antibodies, which have been raised, was found as the best that one against Listeria mo
Czech name
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Czech description
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Project
<a href="/en/project/GA525%2F03%2F0350" target="_blank" >GA525/03/0350: Development of screening immunochemical method for specific detection of Listeria monocytogenes in foods</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
1st International Symposium on Recent Advances in Food Analysis, Book of Abstracts
ISBN
80-7080-528-5
ISSN
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e-ISSN
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Number of pages
1
Pages from-to
39
Publisher name
VŠCHT
Place of publication
FIRMA-JK
Event location
CZ
Event date
Nov 5, 2003
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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