Development of a rapid, simple paddle-style dipstick dye immunoassay specific for Listeria monocytogenes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F06%3A00017221" target="_blank" >RIV/60461373:22330/06:00017221 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Development of a rapid, simple paddle-style dipstick dye immunoassay specific for Listeria monocytogenes
Original language description
We have developed two types of new paddlestyle dipstick dye immunoassays. The first is genus Listeria specific and the second is specific to Listeria monocytogenes. They are based respectively, on antisera raised against heat-killed L. monocytogenes cells and against internalin B crude extract, a virulence protein found only in the pathogenic L. monocytogenes. The minimum detectable level for L. monocytogenes is 2x107 CFU ml−1 for strain number 88/049 in pure culture. Detection is unaffected by the presence of high numbers (approximately log 8.0 CFU/ml) of the other microorganisms tested. When the dipsticks were applied to milk samples inoculated with L. monocytogenes reference material (ALM92), there was a strong response to the enrichment cultures. The new assay may prove useful in detection of L. monocytogenes in enrichment cultures of milk and ice cream food samples.
Czech name
Vývoj rychlé a jednoduché imunoanalýzy specifické pro Listeria monocytogenes s vyuřitím dipstiků
Czech description
Byly vyvinuty dva typy dipstikových imunoanalýz. Jedna je specifická pro Listérie a druhá pro Listeria monocytogenes.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA525%2F03%2F0350" target="_blank" >GA525/03/0350: Development of screening immunochemical method for specific detection of Listeria monocytogenes in foods</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2006
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
European Food Research and Technology
ISSN
1438-2377
e-ISSN
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Volume of the periodical
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Issue of the periodical within the volume
223
Country of publishing house
DE - GERMANY
Number of pages
7
Pages from-to
821-827
UT code for WoS article
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EID of the result in the Scopus database
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