Transgenic Resistin Impair skeletal Muscle Glucose Metabolism in the Spontaneosly Hypertensive Rat

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F03%3A00008965" target="_blank" >RIV/60461373:22330/03:00008965 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Transgenic Resistin Impair skeletal Muscle Glucose Metabolism in the Spontaneosly Hypertensive Rat

Original language description

Increased serum levels of resistin, a molecule secreted by fat cells, have been proposed as a possible mechanistic link between obesity and insulin resistance. To further investigate the effects of resistin on glucose metabolism, we derived a novel transgenic strain of spontaneously hypertensive rats expressing the mouse resistin gene under the control of the fat-specific aP2 promoter and also performed in vitro studies of the effects of recombinant resistin on glucose metabolism in isolated skeletal muscle. Expression of the resistin transgene was detected by Northern blot analysis in adipose tissue and by real-time PCR in skeletal muscle and was associated with increased serum fatty acids and muscle triglycerides, impaired skeletal muscle glucose metabolism, and glucose intolerance in the absence of any changes in serum resistin concentrations. In skeletal muscle isolated from non-transgenic spontaneously hypertensive rats, in vitro incubation with recombinant resistin significantly

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GA301%2F03%2F0751" target="_blank" >GA301/03/0751: Transgenic analysis of the resistin effects on cyrbohydrate and lipid metabolism in spontaneously hypertensive rats</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

The Journal of biological chemistry

ISSN

0021-9258

e-ISSN

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Volume of the periodical

278

Issue of the periodical within the volume

46

Country of publishing house

US - UNITED STATES

Number of pages

7

Pages from-to

45209-15

UT code for WoS article

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EID of the result in the Scopus database

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