Cloning of a bacterial PCB-degrading gene into the plants of Nicotiana tabacum - sborník
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F03%3A00008981" target="_blank" >RIV/60461373:22330/03:00008981 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Cloning of a bacterial PCB-degrading gene into the plants of Nicotiana tabacum - sborník
Original language description
The target of this work is to increase biodegradation of polychlorinated biphenyls (PCB) by plants transformed with the gene bph C originally located in genome of PCB degrading bacteria Comamonas testosteroni B356. The gene bphC encoding the enzyme 2,3-dihydroxybiphenyl-1,2-dioxygenase was cloned in cassette first with the detection marker GFP (green fluorescent protein) to plant cells of Nicotiana tabacum. Transgenic plants proved higher resistence to PCB than nontrasgenic ones. Because of the difficulties with the detection of GFP in plants, caused by autofluorescence of plant tissue, also other constructs were prepared. These contain luciferase gene cloned next to the bphC gene, beta-glucuronidase gene or six histidine motives. Histidine tail allowsfacility isolation of the enzyme. The presence of bphC/GFP DNA and RNA was proved by PCR using various sets of primers. In some of the transformants the fusion protein bphC/GFP was detected by Western blot analysis. Other constructs with
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA526%2F01%2F1292" target="_blank" >GA526/01/1292: Studies on co-operation of plant systems and rhizospheric microorganisms in the metabolism of organic compounds contaminating the environment</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Proceeding of 6th International Symposium and Exhibition on Environmental Contamination in Central and Eastern Europe
ISBN
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ISSN
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e-ISSN
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Number of pages
1
Pages from-to
175
Publisher name
Florida State University
Place of publication
Florida
Event location
Praha
Event date
Sep 1, 2003
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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