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Cloning of a bacterial PCB-degrading gene into the plants of Nicotiana tabacum - sborník

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F03%3A00008981" target="_blank" >RIV/60461373:22330/03:00008981 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Cloning of a bacterial PCB-degrading gene into the plants of Nicotiana tabacum - sborník

  • Original language description

    The target of this work is to increase biodegradation of polychlorinated biphenyls (PCB) by plants transformed with the gene bph C originally located in genome of PCB degrading bacteria Comamonas testosteroni B356. The gene bphC encoding the enzyme 2,3-dihydroxybiphenyl-1,2-dioxygenase was cloned in cassette first with the detection marker GFP (green fluorescent protein) to plant cells of Nicotiana tabacum. Transgenic plants proved higher resistence to PCB than nontrasgenic ones. Because of the difficulties with the detection of GFP in plants, caused by autofluorescence of plant tissue, also other constructs were prepared. These contain luciferase gene cloned next to the bphC gene, beta-glucuronidase gene or six histidine motives. Histidine tail allowsfacility isolation of the enzyme. The presence of bphC/GFP DNA and RNA was proved by PCR using various sets of primers. In some of the transformants the fusion protein bphC/GFP was detected by Western blot analysis. Other constructs with

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA526%2F01%2F1292" target="_blank" >GA526/01/1292: Studies on co-operation of plant systems and rhizospheric microorganisms in the metabolism of organic compounds contaminating the environment</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2003

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    Proceeding of 6th International Symposium and Exhibition on Environmental Contamination in Central and Eastern Europe

  • ISBN

  • ISSN

  • e-ISSN

  • Number of pages

    1

  • Pages from-to

    175

  • Publisher name

    Florida State University

  • Place of publication

    Florida

  • Event location

    Praha

  • Event date

    Sep 1, 2003

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article