A reorientation of helical domains of the matrix protein from Mason-Pfizer monkey virus caused by a single point mutation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F04%3A00012726" target="_blank" >RIV/60461373:22330/04:00012726 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
A reorientation of helical domains of the matrix protein from Mason-Pfizer monkey virus caused by a single point mutation
Original language description
Mason-Pfizer monkey virus (M-PMV) belongs to the family of betaretroviruses (formerly D-type retroviruses) which differ from lentiviruses (formerly C-type viruses) (e. g. HIV-1) in mechanism of immature capsid assembly. Matrix protein (MA), a virus structural protein, plays an essential role in different stages of retrovirus maturation. A single point mutation (R55F) in the sequence of the wt MA, however, changes morphogenesis of the virus and capsids are assembled at the plasma membrane instead of in the cytoplasm, similarly to the C-type retroviruses.We have focused on the study of three-dimensional structure and dynamics of both proteins (wt MA and R55F mutant) by NMR spectroscopy to reveal possible structural changes caused by the mutation (R55F).Although a structure of the wt MA has already been published as C-alpha trace, we decided to perform a new study of this protein to obtain a complete 3D structure. Doubly labelled (13C, 15N) proteins were prepared using recombinant techni
Czech name
Změna orientace helikální domény matrixového proteinu MPMV způsobená mutací jedné aminokyseliny
Czech description
Změna orientace helikální domény matrixového proteinu MPMV způsobená mutací jedné aminokyseliny
Classification
Type
D - Article in proceedings
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
The retrovirus assembly meeting
ISBN
80-86313-13-1
ISSN
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e-ISSN
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Number of pages
1
Pages from-to
49
Publisher name
JPM Tisk s. r. o.
Place of publication
Praha
Event location
Praha
Event date
Oct 2, 2004
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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