Analysis of tryptophan surface accessibility by MALDI-TOF mass spectrometry

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F04%3A00012732" target="_blank" >RIV/60461373:22330/04:00012732 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Analysis of tryptophan surface accessibility by MALDI-TOF mass spectrometry
Original language description
Side chains of surface accessible amino acids play very important role in a biological activities of proteins. They can act as a part of enzyme's active center or participate on many intermolecular interactions. Information about the surface accessibility of selected amino acids can contribute to understanding the protein structure and function. The main interest of this work is to find out whether a specific chemical modification of tryptophan side chain in a molecule of a native protein could reflectssome relevant information about the accessibility and subsequently, information about the protein conformation. The reaction with 2-hydroxy-5-nitrobenzyl bromide (HNB) as a common and highly specific covalent modification of tryptophan seems to be veryuseful for this purposes.This method is based on the side chain modification followed by the analysis of the tryptic digest by MALDI-TOF (matrix-assisted laser desorption/ionization time-of-flight) mass spectrometry. The procedure was tes
Czech name
Analysa povrchové dostupnosti tryptofanů pomocí hmotnostní spektrometrie MALDI-TOF
Czech description
Analysa povrchové dostupnosti tryptofanů pomocí hmotnostní spektrometrie MALDI-TOF

Project
<a href="/en/project/GA203%2F02%2F0922" target="_blank" >GA203/02/0922: Application of MALDI-TOF (matrix assisted laser desorption ionisation - Time of Flight) mass spectrometry on the determination of protein conformation</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Acta Universitatis Palackianae Olomucensis, Facultas Rerum Naturalium, Chemica
ISBN
80-2440882-1
ISSN
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e-ISSN
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Number of pages
1
Pages from-to
34
Publisher name
Univerzita Palackého
Place of publication
Olomouc
Event location
Olomouc, Czech Republic
Event date
Aug 31, 2004
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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