Immunoassay for biochanin A

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F04%3A00012802" target="_blank" >RIV/60461373:22330/04:00012802 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Immunoassay for biochanin A

Original language description

Two variants of immunoassay for determination of biochanin A (5,7-dihydroxy 4´-methoxy isoflavone), i.e. the RIA and the indirect ELISA, have been developed and evaluated. Both methods employ the same rabbit antiserum to a 7-O-carboxymethyl-5-hydroxy-4'-methoxyisoflavone # bovine serum albumin conjugate. A 125I labeled hapten-tyrosine methyl ester conjugate was used as radioligand for the RIA. The indirect ELISA uses immunogen coated microtitration plates and a peroxidase labeled anti-rabbit Ig antibody.Both methods are specific for biochanin A with a comparable sensitivity (3.1 pg/tube for RIA; 5.3 pg/well for ELISA), however their sensitivity to individual crossreactants differs. Main crossreactants are sissotrin (the crossreactivity 15.7% for RIA; 120% for ELISA), 5-hydroxy, 4´,7-dimethoxy isoflavone (51.5%, RIA; 46.5%, ELISA), prunetin (4.5%, RIA; 5.0%, ELISA), genistein (0.8%, RIA; 2.8%, ELISA) and formononetin (0.4%, RIA; 0.3%, ELISA). The method was used for analysis of biochani

Czech name

Imunoanalýza biochaninu A

Czech description

ELISA a RIA metody pro stanovení biochaninu A byly vyvinuty na základě téhož polyklonálního králičího antiséra proti konjugátu biochaninu s hovězím sérovým albuminem. Obě metody jsou srovnatelně specifické a citlivé pro biochanin A (3.1 pg/zkumavku pro RIA; 5.3 pg/jamku pro ELISA), ale liší se v citlivosti vůči některým křížovým reaktantům. Metody byly použity pro stanovení biochaninu A ve vojtěšce a v několika ne-bobovitých rostlinách.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EF - Botany

OECD FORD branch

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Project

<a href="/en/project/GA525%2F03%2F0352" target="_blank" >GA525/03/0352: Isoflavonoids in the Myrtaceae and the Rutaceae families</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Immunological Methods

ISSN

0022-1759

e-ISSN

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Volume of the periodical

Neuveden

Issue of the periodical within the volume

294

Country of publishing house

BE - BELGIUM

Number of pages

9

Pages from-to

155-163

UT code for WoS article

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EID of the result in the Scopus database

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