INFLUENCE OF CYSTEINE RESIDUES ON THE ACTIVITY OF THE PROTEASE OF MASON-PFIZER MONKEY VIRUS.

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F04%3A00012809" target="_blank" >RIV/60461373:22330/04:00012809 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
INFLUENCE OF CYSTEINE RESIDUES ON THE ACTIVITY OF THE PROTEASE OF MASON-PFIZER MONKEY VIRUS.
Original language description
Mason-Pfizer monkey virus assembles immature capsids in the cytoplasm and the temporal and spatial regulation of the maturation is critical, since the PR is not activated until immature capsids reach the cell membrane. Several evidences for a regulationof the activity of retroviral proteases by reversible oxidative modification of the cysteine residues were reported1,2. The structural and functional analysis of a mutant of M-PMV PR, in which two cysteine residues (Cys7, Cys106) were replaced by alanines confirmed, that this mutation decreases the stability, the proteolytic activity of PR, and the propensity of the monomers to form dimers3. Here we present results of a high resolution NMR structure of the inactive mutant (D26N) of the 12 kDa form of M-PMV PR with preserved cysteine residues. The data together with a biochemical characterization, MALDI-TOF analysis revealed the formation of an intramolecular disulphide bridge, which helps to stabilize the monomeric scaffold, and moves t
Czech name
vliv cysteinových zbytků na aktivitu proteasy Mason Pfizerova opičího viru
Czech description
vliv cysteinových zbytků na aktivitu proteasy Mason Pfizerova opičího viru

Project
<a href="/en/project/LN00A079" target="_blank" >LN00A079: Center of Integrated Genomics</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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