Distinct Roles for Nucleic Acid in In Vitro Assembly of Purified Mason-Pfizer Monkey Virus CANC Proteins

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F06%3A00017226" target="_blank" >RIV/60461373:22330/06:00017226 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Distinct Roles for Nucleic Acid in In Vitro Assembly of Purified Mason-Pfizer Monkey Virus CANC Proteins

Original language description

In contrast to other retroviruses, Mason-Pfizer monkey virus (M-PMV) assembles immature capsids in the cytoplasm. We have compared the ability of minimal assembly-competent domains from M-PMV and human immunodeficiency virus type 1 (HIV-1) to assemble invitro into virus-like particles in the presence and absence of nucleic acids. A fusion protein comprised of the capsid and nucleocapsid domains of Gag (CANC) and its N-terminally modified mutant ( ProCANC) were used to mimic the assembly of the viral core and immature particles, respectively. In contrast to HIV-1, where CANC assembled efficiently into cylindrical structures, the same domains of M-PMV were assembly incompetent. The addition of RNA or oligonucleotides did not complement this defect. In contrast, the M-PMV ProCANC molecule was able to assemble into spherical particles, while that of HIV-1 formed both spheres and cylinders. For M-PMV, the addition of purified RNA increased the efficiency with which ProCANC formed spherical

Czech name

Úloha nukleové kyseliny při in vitro skládání částic z CANC proteinu Mason-Pfizerova opičího viru

Czech description

Na rozdíl od jiných retrovirů je Mason-Pfizerův opičí virus schopen skládat své částice v cytoplasmě hostitelské buňky. Srovnávali jsme schopnost minimální domény strukturního polyproteinového prekursoru Gag skládat částice in vitro v přítomnosti i bez nukleové kyseliny. Byly stanoveny množství nukleových kyselin inkorporovaných do částic a na základě výsledků byla stanovena hypotéza o roli nukleové kyseliny při skládání retrovirové částice.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Virology

ISSN

0022-538X

e-ISSN

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Volume of the periodical

2006

Issue of the periodical within the volume

14

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

7089-7099

UT code for WoS article

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EID of the result in the Scopus database

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