In vitro study of assembly of Mason-Pfizer monkey virus CA-NC capsids

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F05%3A00015563" target="_blank" >RIV/60461373:22330/05:00015563 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22330/05:00016398
Result on the web
—
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
In vitro study of assembly of Mason-Pfizer monkey virus CA-NC capsids
Original language description
In contrast to other retroviruses as HIV, Mason-Pfizer monkey virus (M-PMV) preassembles immature capsids in the cytoplasm. We have performed the in vitro assembly of purified bacterially expressed proteins to address the difference of morphogenically distinct retroviruses M-PMV and HIV in their ability to assemble VLPs from minimized assembly competent domains. The fusion capsid and nucleocapsid protein (CANC) and its N-terminally modified mutant ( ProCANC) were used to mimic the assembly of mature andimmature particles, respectively. We found dramatically different ability to assemble CANC particles between these two viruses. Also ProCANC particles of M-PMV differed from that of HIV forming spheres and tubes, respectively. The requirements for factors affecting assembly were also evaluated and the amount of incorporated fluorescently labeled nucleic acids was determined. The presence of purified RNA efficiently promoted formation of ProCANC spherical particles. The oligonucleotides
Czech name
Studie in vitro skládání CA-NC kapsid Mason-Pfizerova opičího viru
Czech description
Studie in vitro skládání CA-NC kapsid Mason-Pfizerova opičího viru

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)