Comparison of Dinitrotoluene Degradation by a Mixed Culture in Aqueous Batch System
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F04%3A00012961" target="_blank" >RIV/60461373:22330/04:00012961 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Comparison of Dinitrotoluene Degradation by a Mixed Culture in Aqueous Batch System
Original language description
A mixed culture enriched from aged nitrotoluenes-contaminated soil from an ammunition plant in the Czech Republic was used to study biodegradation of 2,4-dinitrotoluene (2,4-DNT) and 2,6-dinitrotoluene (2,6-DNT) in aqueous solution under aerobic conditions. The study was conducted in shake flasks containing mineral salt medium. In first set of experiments degradations of the individual 2,4-DNT and 2,6-DNT were tested. The medium was either supplemented with glycerol or with succinate as primary carbon sources. Two different initial DNT concentrations (5.2 or 25 mg.L-1) were used in these experiments. The results proved the total removal of 2,4-DNT after 4 days and 2,6-DNT after 7 days in case of lower starting DNT concentrations and in presence of glycerol. With succinate 2,4-DNT was removed after 10 days but 2,6-DNT was poorly removed.Higher DNT concentrations were also removed better in case of 2,4-DNT but to 50 % after 6 days only. In addition, a metabolite accumulated in the medium
Czech name
Porovnání degradace dinitrotoluenů pomocí směsné populace ve vodném prostředí
Czech description
Směsná populace získana z dlohodobě kontaminované půdy byla použita pro studium degradace 2,4-DNT a 2,6-DNT ve vodném prostředí. Pro pokusy bylo použito BSM medium. Probíhaly na laboratorních třepačkách při 120 rpm. Počáteční koncentrace byly zvoleny 5 a25 mg.L-1. Použití vyšší koncentrace způsobylo degradaci přibližne z 50 % během 6 dnů. Po 6 měsíčním selekčním tlaku byla směsná populace schopna degradovat 2,6-DNT téměř kompletně. V posledním pokusu sloužili 2,4-DNT a 2,6-DNT jako jediný zdroj C, N aenergie. 2,6-DNT byl degradován ze 40 % bez akumulaxce jakéhokoliv metabolitu.
Classification
Type
C - Chapter in a specialist book
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA104%2F04%2F0686" target="_blank" >GA104/04/0686: Microbial mineralization of nitrotoluenes in various reactor systems</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Contaminated soils, sediments and water: Science in the real world
ISBN
0-387-23036-X
Number of pages of the result
15
Pages from-to
91-105
Number of pages of the book
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Publisher name
Springer Science+Business Media, Inc.
Place of publication
New York
UT code for WoS chapter
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