Comparation of Dinitrotoluene Degradation by a Mixed Culture in Aqueous Batch System
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F05%3A00016333" target="_blank" >RIV/60461373:22330/05:00016333 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Comparation of Dinitrotoluene Degradation by a Mixed Culture in Aqueous Batch System
Original language description
A mixed culture, enriched from nitrotoluenes-contaminated aged-soil from an ammunition plant in the Czech Republic, was used to study biodegradation of 2,4-dinitrotoluene (2,4-DNT) and 2,6-dinitrotoluene (2,6-DNT) in aqueous media under aerobic conditions. The study was conducted in shake flasks with mineral salt medium with or without additional carbon and energy source. The mineral salt medium was supplemented with either glycerol or succinate as t he primary carbon and energy source. After six monthsof a selection pressure by DNTs, the mixed culture was still able to degrade 2,4-DNT almost completely; its ability to degrade 2,6-DNT in basal salt medium also increased. Also no intermediates accumulated in the medium. When the dinitrotoluenes were used as the sole source of C-, N- and energy, ~ 40 % of each DNT was removed within 4 days. However, uptake of 2,4-DNT showed a lag phase and one unidentified intermediate accumulated in the medium. 2,6-DNT uptake did not show any lag-phase
Czech name
Porovnání degradace dinitrotoluenu směsnou populací ve vodném batch systému
Czech description
Směsná mikrobní populace získaná z kontaminovaných ploch v České republice byla použita ke studiu degradace 2,4-dinitrotoluenu (2,4-DNT) a 2,6-dinitrotoluenu (2,6-DNT) ve vodní fázi za aerobních podmínek. Pokusy probíhali v baňkách na třepačkách s minerálním médiem s nebo bez dodatečného zdroje uhlíku a energie (glycerol nebo sukcinát). Po šesti měsících selekčního tlaku byla směsná kultura schopná degradovat 2,4-DNT téměř kompletně a její schopnost degradovat 2,6-DNT se zvýšila. Degradace 2,4-DNT vykazovala lag fázi a byl pozorován vznik nekompletně degradovaného intermediátu. Naproti tomu degradace 2,6-DNT nevykazovala lag fázi a nebyl pozorován vznik nekompletně degradovaného intermediátu.
Classification
Type
C - Chapter in a specialist book
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA104%2F04%2F0686" target="_blank" >GA104/04/0686: Microbial mineralization of nitrotoluenes in various reactor systems</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Sediments and Water: Science and the Real World
ISBN
0-387-23036-X
Number of pages of the result
14
Pages from-to
91-104
Number of pages of the book
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Publisher name
Springer Sci. + Business Media Inc.
Place of publication
New York
UT code for WoS chapter
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