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Methods for identification of rhizosperic bacteria degrading PCB and Approach to identify them by molecular biology method

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F04%3A00012984" target="_blank" >RIV/60461373:22330/04:00012984 - isvavai.cz</a>

  • Alternative codes found

    RIV/60461373:22330/04:00019590

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Methods for identification of rhizosperic bacteria degrading PCB and Approach to identify them by molecular biology method

  • Original language description

    This work is based on research which focuses on the study of the possible mutual relationship of microorganisms and plants in PCB metabolism. Three different plant species - Nicotiana tabacum (tobacco), Solanum nigrum (black nightshade) and Medicago sativa (alfalfa) - were cultivated in real soil from an industrial site, which is long-term contaminated by PCBs. After six months the decrease of PCB content in soil and the PCB accumulation in plant tissue was analysed. The highest decrease of PCB concentration was measured from the soil vegetated with tobacco (34% decrease compared to bulk soil). From this polluted soil, bacteria which potentially participate in the biodegradation of PCBs were isolated and were screened for the presence of bphA1 and bphCgenes. These genes are apart of the bph operon, which is very important for PCB degradation. The PCR primers were designed for conserved regions of bphA1 and bphC genes from an alignment of previously published sequences. The degradative

  • Czech name

    Metody identifikace rhisosférních mikroorganosmů degradující PCB

  • Czech description

    Metody identifikace rhisosférních baktérií degradujících PCB za použití molekulárně biologických metod

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    EI - Biotechnology and bionics

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA526%2F01%2F1292" target="_blank" >GA526/01/1292: Studies on co-operation of plant systems and rhizospheric microorganisms in the metabolism of organic compounds contaminating the environment</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2004

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    Conference Rhizoremediace

  • ISBN

  • ISSN

  • e-ISSN

  • Number of pages

    1

  • Pages from-to

    254

  • Publisher name

    Hartmann A.

  • Place of publication

    Mnichov

  • Event location

    Mnichpv

  • Event date

    Jan 1, 2004

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article