PCR protocol for detecting polychlorinated bibphenyl degradation genes in polluted soil
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F04%3A00012996" target="_blank" >RIV/60461373:22330/04:00012996 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
PCR protocol for detecting polychlorinated bibphenyl degradation genes in polluted soil
Original language description
This work is based on research which focuses on the study of the possible mutual relationship of microorganisms and plants in PCB metabolism. Three different plant species - Nicotiana tabacum (tobacco), Solanum nigrum (black nightshade) and Medicago sativa (alfalfa) - were cultivated in real soil from an industrial site, which is long-term contaminated by PCBs. After six months the decrease of PCB content in soil and the PCB accumulation in plant tissue was analysed. The highest decrease of PCB concentration was measured from the soil vegetated with tobacco (34% decrease compared to bulk soil). From this polluted soil, bacteria which potentially participate in the biodegradation of PCBs were isolated and were screened for the presence of bphA1 and bphCgenes. These genes are apart of the bph operon, which is very important for PCB degradation. The PCR primers were designed for conserved regions of bphA1 and bphC genes from an alignment of previously published sequences. The degradative
Czech name
Detekce PCB degradujících bakterií z půdě
Czech description
Detekce PCB degradujících kmenů
Classification
Type
D - Article in proceedings
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA526%2F01%2F1292" target="_blank" >GA526/01/1292: Studies on co-operation of plant systems and rhizospheric microorganisms in the metabolism of organic compounds contaminating the environment</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Proceeding of the 9th Methodological workshop
ISBN
80-86525-0301
ISSN
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e-ISSN
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Number of pages
4
Pages from-to
116-119
Publisher name
Chroňáková, Krištůfek, Elthotová, Malý eds.
Place of publication
ČR
Event location
České Budějovice
Event date
Jan 1, 2004
Type of event by nationality
CST - Celostátní akce
UT code for WoS article
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