Application of flow cytometry to Saccharomyces cerevisiae population analysis-poster

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F05%3A00015491" target="_blank" >RIV/60461373:22330/05:00015491 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Application of flow cytometry to Saccharomyces cerevisiae population analysis-poster
Original language description
This study was focused on a development and an application of a rapid and reliable staining method for characterisation of Saccharomyces cerevisiae cells. Three different fluorescent probes such as propidium iodide (PI), fluorescein diacetate (FDA) and fluorescein isothiocyanate (FITC) were used for staining. PI was used to determine cell viability in a native sample and DNA content in sample fixed by ethanol. To assess protein distribution in yeast population the FITC amine-reactive probe was used. Instantaneous cell enzyme activity was measured as an amount of fluorescein liberated from FDA by intracellular esterase activity.
Czech name
Aplikace průtokové cytometrie v analýze kvasinek Saccharomyces cerevisiae
Czech description
Tato práce byla zaměřena na optimalizaci a aplikaci vhodných metod fluorescenčního značení k charakterizaci buněk Saccharomyces cerevisiae. Využita byla tři různá fluorescenční barviva, propidium jodid, k determinaci buněčné viability a obsahu DNA, fluorescein diacetát k měření okamžité enzymové aktivity a fluorescein isothiokyanát ke sledování obsahu intracelulárních bílkovin.

Project
<a href="/en/project/GA525%2F03%2F0375" target="_blank" >GA525/03/0375: Image analyses of microbial population dynamics</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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