Application of flow cytometry to Saccharomyces cerevisiae population analysis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F05%3A00016340" target="_blank" >RIV/60461373:22330/05:00016340 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22330/05:00019125
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Application of flow cytometry to Saccharomyces cerevisiae population analysis
Original language description
This study was focused on a development and an application of a rapid and reliable staining method for characterisation of Saccharomyces cerevisiae cells. Three different fluorescent probes such as propidium iodide (PI), fluorescein diacetate (FDA) and fluorescein isothiocyanate (FITC) were used for staining. PI was used to determine cell viability in a native sample and DNA content in sample fixed by ethanol. To assess protein distribution in yeast population the FITC amine-reactive probe was used. Instantaneous cell enzyme activity was measured as an amount of fluorescein liberated from FDA by intracellular esterase activity.
Czech name
Aplikace průtokové cytometrie v analýze kvasinek Saccharomyces cerevisiae
Czech description
Tato práce byla zaměřena na optimalizaci a aplikaci vhodných metod fluorescenčního značení k charakterizaci buněk Saccharomyces cerevisiae. Využita byla tři různá fluorescenční barviva, propidium jodid, k determinaci buněčné viability a obsahu DNA, fluorescein diacetát k měření okamžité enzymové aktivity a fluorescein isothiokyanát ke sledování obsahu intracelulárních bílkovin.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chimia
ISSN
0009-4293
e-ISSN
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Volume of the periodical
59
Issue of the periodical within the volume
10
Country of publishing house
CH - SWITZERLAND
Number of pages
4
Pages from-to
745-748
UT code for WoS article
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EID of the result in the Scopus database
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