The puzzling role of nucleic acids in the assembly of Mason-Pfizer monkey virus
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F05%3A00015669" target="_blank" >RIV/60461373:22330/05:00015669 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22330/05:00016396
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
The puzzling role of nucleic acids in the assembly of Mason-Pfizer monkey virus
Original language description
All retroviruses form a structural polyprotein precursor Gag that directs the assembly of the immature retroviral capsid. The role of nucleic acid in the assembly process was extensively studied and several models predicted how the nucleic acid could work and participate on retroviral capsid assembly, but still the exact function of nucleic acid is not clear. We have studied the in vitro assembly process of Mason-Pfizer monkey virus Gag deletion mutant, consisting of two structural proteins, capsid andnucleocapsid. We have focused mainly on (pro-)CANC, CA-NC fusion protein lacking its N-terminal proline, aminoacid that is determining the shape of assembled particles. This mutant assembles with high efficiency in vitro, forming spherical particles of 75 nm diameter in average.To study the role of nucleic acids in the assembly process we optimized the protein purification method that provides the nucleic acid free protein. For studying the assembly process we have used several nucleic a
Czech name
Záhadná role nukleových kyselin v assembly Mason-Pfizerova opičího viru
Czech description
Záhadná role nukleových kyselin v assembly Mason-Pfizerova opičího viru
Classification
Type
O - Miscellaneous
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů