The influence of assembly conditions on effeiciency and morphology of in vitro assembled capsid-like particles formed from CA-NC of Mason-Pfirzer money virus
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F04%3A00012908" target="_blank" >RIV/60461373:22330/04:00012908 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
The influence of assembly conditions on effeiciency and morphology of in vitro assembled capsid-like particles formed from CA-NC of Mason-Pfirzer money virus
Original language description
Domains within structural polyprotein Gag of all retroviruses initiate and direct assembly of the immature viral particles. Gag forms capsid-like particles in bacteria, mammalian cells and in vitro. Several studies have shown that Gag and its deletion mutants, e.g. capsid-nucleocapsid fusion protein (CA-NC) or CA assemble in vitro into spherical, conical or tubular particles. Morphology of formed particles depends on virus type and assembly conditions. CA itself can assemble in vitro into tubular or conical structures resembling a mature core. NC facilitates Gag interactions and it is crucial for specific incorporation of genomic RNA. In previous study we have shown that Mason-Pfizer monkey virus deletion construct consisting of CA and NC fusion protein that lacks the N-terminal proline (Pro-)CA-NC assembles into spherical particles when expressed in E. coli in contrast with tubular structures formed from CA-NC under the same conditions. To evaluate the effect of nucleic acids on the a
Czech name
Vliv podmínek assembly na morfologii částic tvořených z CA-NC proteinu MPMV
Czech description
polyprotein Gag
Classification
Type
D - Article in proceedings
CEP classification
EE - Microbiology, virology
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/LN00A079" target="_blank" >LN00A079: Center of Integrated Genomics</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Virus Assembly Conference
ISBN
—
ISSN
—
e-ISSN
—
Number of pages
1
Pages from-to
254
Publisher name
Vermont Academy
Place of publication
Vermont
Event location
Vermont
Event date
Jan 1, 2004
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
—