Luminometric method for screening retroviral protease inhibitors

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F05%3A00016066" target="_blank" >RIV/60461373:22330/05:00016066 - isvavai.cz</a>
Alternative codes found
RIV/61388963:_____/05:00021541
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Luminometric method for screening retroviral protease inhibitors
Original language description
We have developed a sensitive luminometric assay for determining the activity of retroviral proteases that uses proteolytic cleavage of polypeptide substrate immobilized on Ni-NTA HisSorb Strips microplates. The protease substrate derived from the Gag precursor protein of Mason-Pfizer monkey virus (M-PMV) was conjugated with horseradish peroxidase (HRP), which catalyzes oxidation of luminol in the assay. The cleavage of the substrate was monitored as a decrease in luminescent signal caused by the release of the cleavage product conjugated to HRP. Testing of a set of M-PMV protease inhibitors confirmed that this method is sufficiently sensitive and specific for high-throughput screening of retroviral protease inhibitors
Czech name
luminometrická metoda testování inhibitorů retrovirových proteas
Czech description
Vyvinuli jsme citlivou luminometrickou metodu detekce aktivity retrovirové proteasy s využitím polypeptidových substrátů imobililzovaných na Ni-NTA mikrodestičkách.

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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